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| Content Provider | frontiers |
|---|---|
| Author | Álvarez, Belén Nieto-Pelegrín, Elvira Martínez de la Riva, Paloma Toki, Daisuke Poderoso, Teresa Revilla, Concepción Uenishi, Hirohide Ezquerra, Angel Domínguez, Javier |
| Abstract | CLEC12A has been proposed as a suitable target for delivering antigen to dendritic cells (DCs) to enhance vaccine efficacy both in human and mouse. In this study, we have characterized the porcine homologue of CLEC12A (poCLEC12A). Using new monoclonal antibodies (mAb), raised against its ectodomain, poCLEC12A was found to be expressed on alveolar macrophages, blood conventional type 1 and type 2 DCs and plasmacytoid DCs, but not on monocytes, T cells, B cells or NK cells, in contrast to its human and murine homologues. Western blot analysis showed that in alveolar macrophages this receptor is expressed both as a monomer and a dimer. After binding to DCs, anti- poCLEC12A mAb was efficiently internalized. No significant changes were observed in TNFα or IFNα secretion by plasmacytoid DCs stimulated with either CpGs (ODN2216) or polyinosinic-polycytidylic acid (poly I:C), upon incubation with mAb. These results provide the basis for future investigations aimed to assess the ability of anti-poCLEC12A mAbs to improve vaccine efficacy by targeting antigen to DCs. |
| ISSN | 16643224 |
| DOI | 10.3389/fimmu.2020.00863 |
| Volume Number | 11 |
| Journal | Frontiers in Immunology |
| Language | English |
| Publisher Date | 2020-05-13 |
| Access Restriction | Open |
| Subject Keyword | Dendritic cell Monoclonal antibody C-type lectin Clec12A Pig |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
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