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| Content Provider | frontiers |
|---|---|
| Author | Sun, Dongfeng Gai, Zhibo Wu, Jie Chen, Qingfa |
| Description | BackgroundThe function of angiogenesis-related genes (ARGs) in lung adenocarcinoma (LUAD) remains poorly documented. This study was designed to reveal ARGs in LUAD and related networks.MethodsWe worked with sequencing data and clinical information pertaining to LUAD from public databases. ARGs were retrieved from the HALLMARK_ANGIOGENESIS gene set. Differential analysis and Kaplan–Meier (K–M) analysis were performed to authenticate the ARGs associated with LUAD. Weighted gene correlation network analysis was performed on the mining hub genes linked to the abovementioned genes, and functional enrichment analysis was done. Subsequently, Cox regression analyses were used to construct the prognostic gene. POSTN and microvessel density were detected using immunohistochemistry.ResultsPOSTN, an ARG that was highly expressed in patients with LUAD and was closely associated with their weak overall survival was identified. Differentially expressed genes associated with POSTN were mainly enriched in entries related to the tubulointerstitial system, immune response, and epithelial cells. A positive correlation was demonstrated between POSTN expression and tumor microvessel density in LUAD. Subsequently, a prognostic gene signature was constructed and revealed that 4 genes may predict the survival of LUAD patients. Furthermore, the ESTIMATE and CIBERSORT analyses suggested that our risk scoring system may be implicated in altering the immune microenvironment of patients with LUAD. Fina... |
| Abstract | Angiogenesis is a hallmark of cancer and performs a fundamental role in lung cancer progression. However, the function of angiogenesis-related genes (ARGs) in lung adenocarcinoma (LUAD) remains poorly documented. This study was designed to reveal the action and mechanism of ARGs in LUAD. We worked with sequencing data and clinical information for LUAD from public databases. WGCNA was performed to mining hub genes associated with the above genes and functional enrichment analysis was executed. Cox regression analyses were run to construct prognostic gene labels based on the results of the K-M analysis. POSTN and microvessel density were detected by immunohistochemistry and their correlation was analyzed. Estimation of Stromal and Immune cells were employed to profile alterations in the immune microenvironment. Competing endogenous RNA network was structured based on the prognostic genes. We identified POSTN was closely associated with survival of LUAD patients. We constructed a prognostic gene signature, which could also serve as an independent prognostic marker for LUAD. ESTIMATE and CIBERSORT analyses suggested that our risk scoring system may be implicated in altering the immune microenvironment of LUAD patients. A ceRNA network was constructed based on prognostic genes. The genetic signature of POSTN was implicated in the clinical outcome of LUAD patients. A novel prognostic genes signature associated with ARGs was constructed , which may alter the immune microenvironment and ultimately affecting the survival of LUAD patients. |
| ISSN | 2234943X |
| DOI | 10.3389/fonc.2022.699824 |
| Volume Number | 12 |
| Journal | Frontiers in Oncology |
| Language | English |
| Publisher Date | 2022-06-27 |
| Access Restriction | Open |
| Subject Keyword | Angiogenesis Lung adenocarcinoma (LUAD) Angiogenesis-related genes (ARGs) Prognosis Competing endogenous RNA (CeRNA) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
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