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Chemical shift assignments of calmodulin under standard conditions at neutral pH.
| Content Provider | Europe PMC |
|---|---|
| Author | Bej, Aritra Ames, James B. |
| Abstract | The Ca2+ sensor protein, calmodulin (CaM) is ubiquitously expressed in all cells where it binds to hundreds of different target proteins, including dozens of enzymes, receptors, ion channels and numerous Ca2+ transporters. The only published NMR chemical shift assignments for Ca2+-bound CaM (in the absence of a target) have been determined under acidic conditions: at pH 6.5/310 K (BMRB 6541) and pH 6.3/320 K (BMRB 547). However, some CaM/target complexes are not soluble under these conditions. Also, amide chemical shifts are very sensitive to pH and temperature, which can cause large baseline errors when using the existing chemical shift assignments of free CaM to calculate chemical shift perturbations caused by target binding at neutral pH and physiological temperature. We report complete NMR chemical shift assignments of Ca2+-saturated CaM under a set of standard conditions at neutral pH and 308 K that will enable more accurate chemical shift comparison between free CaM and CaM/target complexes (BMRB 51289). |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC9510097&blobtype=pdf |
| ISSN | 18742718 |
| Journal | Biomolecular Nmr Assignments [Biomol NMR Assign] |
| Volume Number | 16 |
| DOI | 10.1007/s12104-022-10082-7 |
| PubMed Central reference number | PMC9510097 |
| Issue Number | 2 |
| PubMed reference number | 35460468 |
| e-ISSN | 1874270X |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2022-04-23 |
| Publisher Place | Dordrecht |
| Access Restriction | Open |
| Rights License | Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/ © The Author(s) 2022 |
| Subject Keyword | CaM Calcium EF-hand Chemical shift perturbation NMR |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Biochemistry |
