NDLI: In Situ-Forming Collagen/poly-γ-glutamic Acid Hydrogel System with Mesenchymal Stem Cells and Bone Morphogenetic Protein-2 for Bone Tissue Regeneration in a Mouse Calvarial Bone Defect Model.

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In Situ-Forming Collagen/poly-γ-glutamic Acid Hydrogel System with Mesenchymal Stem Cells and Bone Morphogenetic Protein-2 for Bone Tissue Regeneration in a Mouse Calvarial Bone Defect Model.

Content Provider	Europe PMC
Author	Cho, Sun-Hee Shin, Keun Koo Kim, Sun-Young Cho, Mi Young Oh, Doo-Byoung Lim, Yong Taik
Abstract	Background: Bone marrow-derived mesenchymal stem cells (BMSCs) and bone morphogenetic protein-2 (BMP-2) have been studied for bone repair because they have regenerative potential to differentiate into osteoblasts. The development of injectable and in situ three-dimensional (3D) scaffolds to proliferate and differentiate BMSCs and deliver BMP-2 is a crucial technology in BMSC-based tissue engineering. Methods: The proliferation of mouse BMSCs (mBMSCs) in collagen/poly-γ-glutamic acid (Col/γ-PGA) hydrogel was evaluated using LIVE/DEAD and acridine orange and propidium iodide assays. In vitro osteogenic differentiation and the gene expression level of Col/γ-PGA(mBMSC/BMP-2) were assessed by alizarin red S staining and quantitative reverse-transcription polymerase chain reaction. The bone regeneration effect of Col/γ-PGA(mBMSC/BMP-2) was evaluated in a mouse calvarial bone defect model. The cranial bones of the mice were monitored by micro-computed tomography and histological analysis. Results: The developed Col/γ-PGA hydrogel showed low viscosity below ambient temperature, while it provided a high elastic modulus and viscous modulus at body temperature. After gelation, the Col/γ-PGA hydrogel showed a 3D and interconnected porous structure, which helped the effective proliferation of BMSCs with BMP-2. The Col/γ-PGA (mBMSC/BMP-2) expressed more osteogenic genes and showed effective orthotopic bone formation in a mouse model with a critical-sized bone defect in only 3–4 weeks. Conclusion: The Col/γ-PGA(mBMSC/BMP-2) hydrogel was suggested to be a promising platform by combining collagen as a major component of the extracellular matrix and γ-PGA as a viscosity reducer for easy handling at room temperature in BMSC-based bone tissue engineering scaffolds. Supplementary Information The online version contains supplementary material available at 10.1007/s13770-022-00454-4.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC9477999&blobtype=pdf
ISSN	17382696
Volume Number	19
DOI	10.1007/s13770-022-00454-4
PubMed Central reference number	PMC9477999
Issue Number	5
PubMed reference number	35460494
Journal	Tissue Engineering and Regenerative Medicine [Tissue Eng Regen Med]
e-ISSN	22125469
Language	English
Publisher	Springer Nature Singapore
Publisher Date	2022-04-23
Publisher Place	Singapore
Access Restriction	Open
Rights License	© Korean Tissue Engineering and Regenerative Medicine Society 2022
Subject Keyword	Hydrogel scaffold Bone morphogenetic protein-2 Mesenchymal stem cell Calvarial defect Bone regeneration
Content Type	Text
Resource Type	Article
Subject	Medicine Biomedical Engineering

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