NDLI: Characterizing Organelles in Live Stem Cells Using Label-Free Optical Diffraction Tomography.

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Characterizing Organelles in Live Stem Cells Using Label-Free Optical Diffraction Tomography.

Content Provider	Europe PMC
Author	Kim, Youngkyu Kim, Tae-Keun Shin, Yeonhee Tak, Eunyoung Song, Gi-Won Oh, Yeon-Mok Kim, Jun Ki Pack, Chan-Gi
Copyright Year	2021
Abstract	Label-free optical diffraction tomography (ODT), an imaging technology that does not require fluorescent labeling or other pre-processing, can overcome the limitations of conventional cell imaging technologies, such as fluorescence and electron microscopy. In this study, we used ODT to characterize the cellular organelles of three different stem cells—namely, human liver derived stem cell, human umbilical cord matrix derived mesenchymal stem cell, and human induced pluripotent stem cell—based on their refractive index and volume of organelles. The physical property of each stem cell was compared with that of fibroblast. Based on our findings, the characteristic physical properties of specific stem cells can be quantitatively distinguished based on their refractive index and volume of cellular organelles. Altogether, the method employed herein could aid in the distinction of living stem cells from normal cells without the use of fluorescence or specific biomarkers.
ISSN	10168478
Journal	Molecules and Cells
Volume Number	44
PubMed Central reference number	PMC8627838
Issue Number	11
PubMed reference number	34819398
e-ISSN	02191032
DOI	10.14348/molcells.2021.0190
Language	English
Publisher	Korean Society for Molecular and Cellular Biology
Publisher Date	2021-11-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ © The Korean Society for Molecular and Cellular Biology. All rights reserved.
Subject Keyword	high density vesicle human stem cell optical diffraction tomography organelle volume three-dimensional quantification
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Molecular Biology

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