NDLI: Label-free high-resolution 3-D imaging of gold nanoparticles inside live cells using optical diffraction tomography

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Label-free high-resolution 3-D imaging of gold nanoparticles inside live cells using optical diffraction tomography

Content Provider	bioRxiv
Author	Kim, Doyeon Oh, Nuri Kim, Kyoohyun Lee, Sangyun Pack, Chan-gi Park, Ji-ho Park, Yongkeun
Copyright Year	2017
Abstract	Abstract Delivery of gold nanoparticles (GNPs) into live cells has high potentials, ranging from molecular-specific imaging, photodiagnostics, to photothermal therapy. However, studying the long-term dynamics of cells with GNPs using conventional fluorescence techniques suffers from phototoxicity and photobleaching. Here, we present a method for 3-D imaging of GNPs inside live cells exploiting refractive index (RI) as imaging contrast. Employing optical diffraction tomography, 3-D RI tomograms of live cells with GNPs are precisely measured for an extended period with sub-micrometer resolution. The locations and contents of GNPs in live cells are precisely addressed and quantified due to their distinctly high RI values, which was validated by confocal fluorescence imaging of fluorescent dye conjugated GNPs. In addition, we perform quantitative imaging analysis including the segmentations of GNPs in the cytosol, the volume distributions of aggregated GNPs, and the temporal evolution of GNPs contents in HeLa and 4T1 cells. Abbreviations GNPs: gold nanoparticles RI: refractive index ODT: optical diffraction tomography DMD: digital micromirror device
Related Links	https://www.biorxiv.org/content/biorxiv/early/2017/05/22/097113.full.pdf
DOI	10.1101/097113
Language	English
Publisher	Cold Spring Harbor Laboratory
Publisher Date	2017-05-22
Access Restriction	Open
Rights License	Creative Commons License (Attribution 4.0 International)
Subject Keyword	Biophysics
Content Type	Text
Resource Type	Preprint
Subject	Biophysics

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