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Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay.
| Content Provider | Europe PMC |
|---|---|
| Author | Batson, Joshua Dudas, Gytis Haas-Stapleton, Eric Kistler, Amy L Li, Lucy M Logan, Phoenix Ratnasiri, Kalani Retallack, Hanna |
| Editor | Soldati-Favre, Dominique |
| Copyright Year | 2021 |
| Abstract | Mosquitoes are major infectious disease-carrying vectors. Assessment of current and future risks associated with the mosquito population requires knowledge of the full repertoire of pathogens they carry, including novel viruses, as well as their blood meal sources. Unbiased metatranscriptomic sequencing of individual mosquitoes offers a straightforward, rapid, and quantitative means to acquire this information. Here, we profile 148 diverse wild-caught mosquitoes collected in California and detect sequences from eukaryotes, prokaryotes, 24 known and 46 novel viral species. Importantly, sequencing individuals greatly enhanced the value of the biological information obtained. It allowed us to (a) speciate host mosquito, (b) compute the prevalence of each microbe and recognize a high frequency of viral co-infections, (c) associate animal pathogens with specific blood meal sources, and (d) apply simple co-occurrence methods to recover previously undetected components of highly prevalent segmented viruses. In the context of emerging diseases, where knowledge about vectors, pathogens, and reservoirs is lacking, the approaches described here can provide actionable information for public health surveillance and intervention decisions. |
| Journal | eLife |
| Volume Number | 10 |
| PubMed Central reference number | PMC8110308 |
| PubMed reference number | 33904402 |
| e-ISSN | 2050084X |
| DOI | 10.7554/elife.68353 |
| Language | English |
| Publisher | eLife Sciences Publications, Ltd |
| Publisher Date | 2021-04-27 |
| Access Restriction | Open |
| Rights License | This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. © 2021, Batson et al |
| Subject Keyword | viral discovery epidemiology microbiota mosquito blood meal reservoir xenosurveillance Virus |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Microbiology Neuroscience Medicine Biochemistry, Genetics and Molecular Biology |
