Loading...
Please wait, while we are loading the content...
Similar Documents
RNA-dependent RNA Polymerase Assay for Hepatitis E Virus.
| Content Provider | Europe PMC |
|---|---|
| Author | Nair, Vidya P. Anang, Saumya Srivastava, Akriti Surjit, Milan |
| Copyright Year | 2017 |
| Abstract | RNA-dependent RNA polymerase (RdRp) is essential for the replication of viral RNA for RNA viruses. It synthesizes the complementary strand of viral genomic RNA, which is used subsequently as a template to generate more copies of viral genome. This assay measures activity of the hepatitis E virus (HEV) RdRp. In contrast to protocols available to assay the RdRp activity of many other viruses, this assay utilizes DIG-11-UTP as a nonradioactive alternative to 32P-UTP, thereby increasing the convenience of performing the assay. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8410430&blobtype=pdf |
| Volume Number | 7 |
| PubMed Central reference number | PMC8410430 |
| Issue Number | 7 |
| PubMed reference number | 34541210 |
| Journal | Bio-protocol [Bio Protoc] |
| e-ISSN | 23318325 |
| DOI | 10.21769/bioprotoc.2199 |
| Language | English |
| Publisher | Bio-Protocol |
| Publisher Date | 2017-04-05 |
| Publisher Place | 1075 Lorne Way, Sunnyvale, CA 94087, USA |
| Access Restriction | Open |
| Rights License | Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC. |
| Subject Keyword | Hepatitis E virus RNA virus RdRp RdRp assay Viral replication Nonradioactive RdRp assay |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Microbiology Neuroscience Plant Science Biochemistry, Genetics and Molecular Biology |
