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Correlation of HLA-DQ and TNF-α gene polymorphisms with ocular myasthenia gravis combined with thyroid-associated ophthalmopathy.
| Content Provider | Europe PMC |
|---|---|
| Author | Yang, Hong-Wei Wang, Ying-Xue Bao, Jie Wang, Shu-Hui Lei, Ping Sun, Zhao-Lin |
| Copyright Year | 2017 |
| Abstract | The present study aims to explore the correlation of human leucocyte antigen (HLA)-DQ and tumour necrosis factor (TNF)-α gene polymorphisms with ocular myasthenia gravis (OMG) combined with thyroid-associated ophthalmopathy (TAO). From March 2009 to March 2015, 56 OMG patients complicated with TAO (OMG + TAO group), 134 patients diagnosed with OMG only (OMG group) and 236 healthy individuals (control group) were enrolled in the present study. PCR-sequence specific primer (PCR-SSP) was used for HLA-DQ genotyping and PCR-restriction fragment length polymorphism (PCR-RFLP) for TNF-α genotyping. ELISA kit was applied to detect acetylcholine receptor antibody (AchRAb) level and chemiluminescence immunoassay (CLIA) to measure thyroid-associated antibody (T-Ab) level. Logistic regression analysis was carried out to analyse the risk factors for OMG combined with TAO. DQA1*0103 showed lower frequency in the OMG group than in the control group. DQA1*0301 showed increased and DQB1*0601 showed decreased frequency in the OMG + TAO group. DQB1*0501 showed higher frequency in the OMG and OMG + TAO groups than in the control group. Patients carrying TNF-α -863C > A (CA + AA) might confront with greater risks of OMG combined with TAO. Frequency of DQA1*0103/*0301 and DQB1*0501/*0601, and TNF-α -863C > A, -238G > A and -308G > A were associated with the levels of AchRAb and T-Ab. TNF-α -863C > A (CA + AA) and high level of T-Ab were risk factors for OMG combined with TAO. Our results demonstrate that TNF-α -863 polymorphism is possibly correlated with the risk of OMG combined with TAO. |
| Page Count | 12 |
| ISSN | 01448463 |
| Volume Number | 37 |
| PubMed Central reference number | PMC5469324 |
| Issue Number | 2 |
| PubMed reference number | 28119492 |
| Journal | Bioscience Reports [Biosci. Rep] |
| e-ISSN | 15734935 |
| DOI | 10.1042/BSR20160440 |
| Language | English |
| Publisher | Portland Press Ltd. |
| Publisher Date | 2017-03-27 |
| Access Restriction | Open |
| Rights License | This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). © 2017 The Author(s). |
| Subject Keyword | Antibody Gene polymorphism HLA-DQ Ocular myasthenia gravis TNF-α Thyroid associated ophthalmopathy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology Biochemistry Biophysics |
