Loading...
Please wait, while we are loading the content...
Similar Documents
CXCR7/CXCL12 axis is involved in lymph node and liver metastasis of gastric carcinoma.
| Content Provider | Europe PMC |
|---|---|
| Author | Xin, Qi Zhang, Na Yu, Hai-Bo Zhang, Qin Cui, Yan-Fen Zhang, Chuan-Shan Ma, Zhe Yang, Yan Liu, Wei |
| Copyright Year | 2017 |
| Abstract | AIMTo investigate the role of CXC chemokine receptor (CXCR)-7 and CXCL12 in lymph node and liver metastasis of gastric carcinoma.METHODSIn 160 cases of gastric cancer, the expression of CXCR7 and CXCL12 in tumor and matched tumor-adjacent non-cancer tissues, in the lymph nodes around the stomach and in the liver was detected using immunohistochemistry to analyze the relationship between CXCR7/CXCL12 expression and clinicopathological features and to determine whether CXCR7 and CXCL12 constitute a biological axis to promote lymph node and liver metastasis of gastric cancer. Furthermore, the CXCR7 gene was silenced and overexpressed in human gastric cancer SGC-7901 cells, and cell proliferation, migration and invasiveness were measured by the MTT, wound healing and Transwell assays, respectively.RESULTSCXCR7 expression was up-regulated in gastric cancer tissues (P = 0.011). CXCR7/CXCL12 expression was significantly related to high tumor stage and lymph node (r = 0.338, P = 0.000) and liver metastasis (r = 0.629, P = 0.000). The expression of CXCL12 in lymph node and liver metastasis was higher than that in primary gastric cancer tissues (χ2 = 6.669, P = 0.010; χ2 = 25379, P = 0.000), and the expression of CXCL12 in lymph node and liver metastasis of gastric cancer was consistent with the positive expression of CXCR7 in primary gastric cancer (r = 0.338, P = 0.000; r = 0.629, P = 0.000). Overexpression of the CXCR7 gene promoted cell proliferation, migration and invasion. Silencing of the CXCR7 gene suppressed SGC-7901 cell proliferation, migration and invasion. Human gastric cancer cell lines expressed CXCR7 and showed vigorous proliferation and migratory responses to CXCL12.CONCLUSIONThe CXCR7/CXCL12 axis is involved in lymph node and liver metastasis of gastric cancer. CXCR7 is considered a potential therapeutic target for the treatment of gastric cancer. |
| ISSN | 10079327 |
| Volume Number | 23 |
| PubMed Central reference number | PMC5423042 |
| Issue Number | 17 |
| PubMed reference number | 28533662 |
| Journal | World Journal of Gastroenterology [World J. Gastroenterol] |
| e-ISSN | 22192840 |
| DOI | 10.3748/wjg.v23.i17.3053 |
| Language | English |
| Publisher | Baishideng Publishing Group Inc |
| Publisher Date | 2017-05-01 |
| Access Restriction | Open |
| Rights License | This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Subject Keyword | Gastric cancer Lymph node metastasis Stromal cell derived factor-1 Liver metastasis CXC chemokine receptor-7 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Gastroenterology |
