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Morus alba Accumulates Reactive Oxygen Species to Initiate Apoptosis via FOXO-Caspase 3-Dependent Pathway in Neuroblastoma Cells.
| Content Provider | Europe PMC |
|---|---|
| Author | Kwon, Young Hwi Bishayee, Kausik Rahman, Ataur Hong, Jae Seung Lim, Soon-Sung Huh, Sung-Oh |
| Copyright Year | 2015 |
| Abstract | Morus alba root extract (MARE) has been used to treat hyperglycaemic conditions in oriental medicine. Here, we studied whether MARE possesses a cytotoxic effect on neuroblastoma. To check the cytotoxicity generated by MARE was whether relatively higher against the cancer cells rather than normal cells, we chose a neuroblastoma cell line (B103) and a normal cell line (Rat-2). A CCK assay revealed that MARE (10 μg/ml) reduced cell viability to approximately 60% compared to an untreated control in B103 cells. But in Rat-2 cells, MARE induced relatively lower cytotoxicity. To investigate the mechanisms underlying the cytotoxic effect of MARE, we used flow cytometry combined with immunoblot analyses. We found that MARE-treatment could accumulate ROS and depolarize mitochondria membrane potential of B103 cells. Further treatment with MARE in B103 cells also could damage DNA and induce apoptosis. An expression study of p-Akt also suggested that there was a reduction in cellular proliferation and transcription along with the process of apoptosis, which was further evidenced by an increase in Bax and cleaved-caspase 3 activity. Together, our findings suggest that MARE produces more cytotoxicity in cancer cells while having a relatively attenuated effect on normal cells. As such, MARE may be a safer option in cancer therapeutics, and it also shows potential for the patients with symptoms of hyperglycemia and cancer. |
| ISSN | 10168478 |
| Journal | Molecules and Cells |
| Volume Number | 38 |
| PubMed Central reference number | PMC4507029 |
| Issue Number | 7 |
| PubMed reference number | 25921607 |
| e-ISSN | 02191032 |
| DOI | 10.14348/molcells.2015.0030 |
| Language | English |
| Publisher | Korean Society for Molecular and Cellular Biology |
| Publisher Date | 2015-07-03 |
| Access Restriction | Open |
| Rights License | This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/. © The Korean Society for Molecular and Cellular Biology. All rights reserved. |
| Subject Keyword | cytotoxicity intrinsic cell death pathway neuroblastoma proliferation reactive oxygen species |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Molecular Biology |
