NDLI: 7-ketocholesterol induces apoptosis of MC3T3-E1 cells associated with reactive oxygen species generation, endoplasmic reticulum stress and caspase-3/7 dependent pathway

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7-ketocholesterol induces apoptosis of MC3T3-E1 cells associated with reactive oxygen species generation, endoplasmic reticulum stress and caspase-3/7 dependent pathway

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Yuta Sato Noriko Ishihara Daiji Nagayama Atsuhito Saiki Ichiro Tatsuno
Abstract	Type 2 diabetes mellitus (T2DM) is associated with an increased risk of bone fractures without reduction of bone mineral density. The cholesterol oxide 7-ketocholesterol (7KCHO) has been implicated in numerous diseases such as atherosclerosis, Alzheimer's disease, Parkinson's disease, cancer, age-related macular degeneration and T2DM. In the present study, 7KCHO decreased the viability of MC3T3-E1 cells, increased reactive oxygen species (ROS) production and apoptotic rate, and upregulated the caspase-3/7 pathway. Furthermore, these effects of 7KCHO were abolished by pre-incubation of the cells with N-acetylcysteine (NAC), an ROS inhibitor. Also, 7KCHO enhanced the mRNA expression of two endoplasmic reticulum (ER) stress markers; CHOP and GRP78, in MC3T3-E1 cells. Pre-incubation of the cells with NAC suppressed the 7KCHO-induced upregulation of CHOP, but not GRP78. In conclusion, we demonstrated that 7KCHO induced apoptosis of MC3T3-E1 cells associated with ROS generation, ER stress, and caspase-3/7 activity, and the effects of 7KCHO were abolished by the ROS inhibitor NAC. These findings may provide new insight into the relationship between oxysterol and pathophysiology of osteoporosis seen in T2DM.
Related Links	http://www.sciencedirect.com/science/article/pii/S2214426916301161
ISSN	22144269
DOI	10.1016/j.ymgmr.2017.01.006
Journal	Molecular Genetics and Metabolism Reports
Issue Number	C
Volume Number	10
Language	English
Publisher	Elsevier
Publisher Date	2017-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Medicine (General) Biology (General) 7-ketocholesterol Mc3t3-e1 Cells Reactive Oxygen Species (ros) N-acetylcysteine (nac) Apoptosis
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Endocrinology

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