NDLI: Aggregatibacter actinomycetemcomitans Invasion Induces Interleukin-1β Production Through Reactive Oxygen Species and Cathepsin B.

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Aggregatibacter actinomycetemcomitans Invasion Induces Interleukin-1β Production Through Reactive Oxygen Species and Cathepsin B.

Content Provider	Europe PMC
Author	Okinaga, Toshinori Ariyoshi, Wataru Nishihara, Tatsuji
Copyright Year	2015
Abstract	Interleukin-1 (IL-1) cytokines, IL-1α, IL-1β, and IL-18 play a crucial role in inflammatory responses in a variety of diseases including periodontitis. In this study, the periodontopathic bacterial pathogen, Aggregatibacter actinomycetemcomitans, induced cell death and cytokine release in macrophages. Cell viability was reduced by A. actinomycetemcomitans invasion using (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay. The production of IL-1β in A. actinomycetemcomitans-invaded macrophage cells was detected by real-time reverse transcriptase–polymerase chain reaction, western blotting, and enzyme-linked immunosorbent assay. Treatment with a caspase-1 inhibitor and silencing of the caspase-1 gene had no effect on IL-1β secretion induced by A. actinomycetemcomitans invasion. Pattern recognition receptor, NLRP3 was upregulated in A. actinomycetemcomitans-invaded macrophages. However, NLRP3 knockdown had no effect on the secretion of IL-1β in A. actinomycetemcomitans-invaded RAW 264 cells. In addition, A. actinomycetemcomitans invasion induced the generation of reactive oxygen species (ROS) and the release of cathepsin B in RAW 264 cells. Interestingly, CA074-Me, a cathepsin B inhibitor, and N-Acetyl-l-cysteine, a ROS inhibitor, prevented the production of IL-1β induced by A. actinomycetemcomitans. Taken together, these results suggest A. actinomycetemcomitans induce IL-1β production in RAW 264 cells through the production of ROS and cathepsin B, but not through the NLRP3/caspase-1 pathway.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4490745&blobtype=pdf
Page Count	10
ISSN	10799907
Volume Number	35
DOI	10.1089/jir.2014.0127
PubMed Central reference number	PMC4490745
Issue Number	6
PubMed reference number	25789553
Journal	Journal of Interferon & Cytokine Research [J Interferon Cytokine Res]
e-ISSN	15577465
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2015-03-19
Publisher Place	USA
Access Restriction	Open
Rights License	Copyright 2015, Mary Ann Liebert, Inc.
Content Type	Text
Resource Type	Article
Subject	Cell Biology Virology Medicine Immunology

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