NDLI: Aggregatibacter actinomycetemcomitans leukotoxin cytotoxicity occurs through bilayer destabilization

Content Provider	PubMed Central
Author	Brown, Angela C. Kathleen, Boesze-battaglia Du, Yurong Stefano, Frank P. Kieba, Irene R. Epand, Raquel F. Kakalis, Lazaros Yeagle, Philip L. Epand, Richard M. Lally, Edward T.
Copyright Year	2012
Abstract	The Gram-negative bacterium, Aggregatibacter actinomycetemcomitans, is a common inhabitant of the human upper aerodigestive tract. The organism produces an RTX (Repeats in ToXin) toxin (LtxA) that kills human white blood cells. LtxA is believed to be a membrane-damaging toxin, but details of the cell surface interaction for this and several other RTX toxins have yet to be elucidated. Initial morphological studies suggested that LtxA was bending the target cell membrane. Because the ability of a membrane to bend is a function of its lipid composition, we assessed the proficiency of LtxA to release of a fluorescent dye from a panel of liposomes composed of various lipids. Liposomes composed of lipids that form nonlamellar phases were susceptible to LtxA-induced damage while liposomes composed of lipids that do not form non-bilayer structures were not. Differential scanning calorimetry demonstrated that the toxin decreased the temperature at which the lipid transitions from a bilayer to a nonlamellar phase, while 31P nuclear magnetic resonance studies showed that the LtxA-induced transition from a bilayer to an inverted hexagonal phase occurs through the formation of an isotropic intermediate phase. These results indicate that LtxA cytotoxicity occurs through a process of membrane destabilization.
Related Links	http://dx.doi.org/10.1111/j.1462-5822.2012.01762.x
Ending Page	881
Page Count	13
Starting Page	869
File Format	PDF
ISSN	14625814
e-ISSN	14625822
Journal	Cellular microbiology
Issue Number	6
Volume Number	14
Language	English
Publisher Date	2012-06-01
Access Restriction	Open
Subject Keyword	Immunology Microbiology Virology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology

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