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Anticancer effect of the extracts from Polyalthia evecta against human hepatoma cell line (HepG2).
| Content Provider | Europe PMC |
|---|---|
| Author | Machana, Sasipawan Weerapreeyakul, Natthida Barusrux, Sahapat |
| Copyright Year | 2012 |
| Abstract | Objective To investigate the anticancer activity of Polyalthia evecta (P. evecta) (Pierre) Finet & Gagnep against human hepatoma cell line (HepG2). Methods The anticancer activity was based on (a) the cytotoxicity against human hepatoma cells (HepG2) assessed using a neutral red assay and (b) apoptosis induction determined by evaluation of nuclei morphological changes after DAPI staining. Preliminary phytochemical analysis of the crude extract was assessed by HPLC analysis. Results The 50% ethanol-water crude leaf extract of P. evecta (EW-L) showed greater potential anticancer activity with high cytotoxicity [IC50 = (62.8 ± 7.3)µg/mL] and higher selectivity in HepG2 cells than normal Vero cells [selective index (SI) = 7.9]. The SI of EW-L was higher than the positive control, melphalan (SI = 1.6) and the apoptotic cells (46.4 ± 2.6) % induced by EW-L was higher than the melphalan (41.6 ± 2.1)% (P<0.05). The HPLC chromatogram of the EW-L revealed the presence of various kinds of polyphenolics and flavonoids in it. Conclusions P. evecta is a potential plant with anticancer activity. The isolation of pure compounds and determination of the bioactivity of individual compounds will be further performed. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3609308&blobtype=pdf |
| ISSN | 22211691 |
| Volume Number | 2 |
| PubMed Central reference number | PMC3609308 |
| Issue Number | 5 |
| PubMed reference number | 23569932 |
| Journal | Asian Pacific Journal of Tropical Biomedicine [Asian Pac J Trop Biomed] |
| DOI | 10.1016/s2221-1691(12)60058-6 |
| Language | English |
| Publisher | Asian Pacific Tropical Medicine Press |
| Publisher Date | 2012-05-01 |
| Access Restriction | Open |
| Rights License | © 2012 by the Asian Pacific Journal of Tropical Biomedicine. All rights reserved. |
| Subject Keyword | Polyalthia evecta Apoptosis induction Cytotoxicity Anticancer activity Hepatoma |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
