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Antioxidant activity and apoptotic induction as mechanisms of action of Withania somnifera (Ashwagandha) against a hepatocellular carcinoma cell line
| Content Provider | SAGE Publishing |
|---|---|
| Author | Ahmed, Wafaa Mofed, Dina Zekri, Abdel-Rahman El-Sayed, Nasr Rahouma, Mohamed Sabet, Salwa |
| Copyright Year | 2018 |
| Abstract | ObjectiveTo evaluate the antioxidant and apoptotic inductive effects of Withania somnifera (Ashwagandha) leaf extract against a hepatocellular carcinoma cell line.MethodsAfter treating HepG2cells with Ashwagandha water extract (ASH-WX; 6.25 mg/ml–100 mg/ml), cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Antioxidant activities (total antioxidant, glutathione S-transferase and glutathione reductase), Fas-ligand level, tumour necrosis factor-α (TNF-α) level and caspase-3, -8, and -9 activities were measured. Molecular modelling assessed the binding-free energies of Ashwagandha in the cyclin D1 receptor.ResultsThe MTT assay demonstrated increased cytotoxicity following treatment of HepG2 cells with ASH-WX compared with control untreated cells and theIC50was 5% (approximately 5.0 mg/ml). Antioxidant activities, Fas-ligand levels and caspase-3, -8 and -9 activities significantly increased, while TNF-α level significantly decreased following ASH-WX treatment compared with control untreated cells. Molecular docking analysis revealed a good prediction of binding between cyclin D1 and Ashwagandha. There was significant accumulation of ASH-WX-treated HepG2cells in the G0/G1 and G2/M phases compared with the control untreated cells.ConclusionAshwagandha could be a powerful antioxidant and a promising anticancer agent against HCC. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/0300060517752022?download=true |
| Starting Page | 1358 |
| Ending Page | 1369 |
| Page Count | 12 |
| ISSN | 03000605 |
| Issue Number | 4 |
| Volume Number | 46 |
| Journal | Journal of International Medical Research (IMR) |
| e-ISSN | 14732300 |
| DOI | 10.1177/0300060517752022 |
| Language | English |
| Publisher | Sage Publications UK |
| Publisher Date | 2018-02-02 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2018 |
| Subject Keyword | antioxidants Ashwagandha apoptosis HepG2 cytotoxicity |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Cell Biology Biochemistry |
