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Cloning, purification, crystallization and preliminary crystallographic study of calcium-binding protein 5 from Entamoeba histolytica.
| Content Provider | Europe PMC |
|---|---|
| Author | Kumar, Sanjeev Zaidi, Rana Gourinath, Samudrala |
| Copyright Year | 2012 |
| Description | Calcium-binding protein 5 from E. histolytica was cloned, expressed in E. coli and purified. The purified protein crystallized in space group C222 and the crystals diffracted to 2 Å resolution. Entamoeba histolytica is the causative agent of human amoebiasis. Phagocytosis is the major route of food intake by this parasite and is responsible for its virulence. Calcium and calcium-binding proteins play major roles in its phagocytosis. Calcium-binding protein 5 from E. histolytica (EhCaBP5) is a cytoplasmic protein; its expression is very sensitive to serum starvation and it seems to be involved in binding to myosin I. In this study, EhCaBP5 was cloned, expressed in Escherichia coli and purified using affinity and size-exclusion chromatography. The purified protein crystallized in space group C222 and the crystals diffracted to 2 Å resolution. The Matthews coefficient indicated the presence of one molecule in the asymmetric unit, with a V M of 2.35 Å3 Da−1 and a solvent content of 47.7%. Entamoeba histolytica is the causative agent of human amoebiasis. Phagocytosis is the major route of food intake by this parasite and is responsible for its virulence. Calcium and calcium-binding proteins play major roles in its phagocytosis. Calcium-binding protein 5 from E. histolytica (EhCaBP5) is a cytoplasmic protein; its expression is very sensitive to serum starvation and it seems to be involved in binding to myosin I. In this study, EhCaBP5 was cloned, expressed in Escherichia coli and purified using affinity and size-exclusion chromatography. The purified protein crystallized in space group C222 and the crystals diffracted to 2 Å resolution. The Matthews coefficient indicated the presence of one molecule in the asymmetric unit, with a VM of 2.35 Å3 Da−1 and a solvent content of 47.7%. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3509984&blobtype=pdf |
| Volume Number | 68 |
| PubMed Central reference number | PMC3509984 |
| Issue Number | Pt 12 |
| Issue Number | pt 12 |
| PubMed reference number | 23192043 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications [Acta Crystallogr Sect F Struct Biol Cryst Commun] |
| e-ISSN | 17443091 |
| DOI | 10.1107/s1744309112044612 |
| Language | English |
| Publisher | International Union of Crystallography |
| Publisher Date | 2012-11-19 |
| Access Restriction | Open |
| Rights License | © International Union of Crystallography 2012 |
| Subject Keyword | calcium-binding protein 5 Entamoeba histolytica |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology |
