NDLI: Crystallization and preliminary crystallographic analysis of cysteine synthase from Entamoeba histolytica.

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Crystallization and preliminary crystallographic analysis of cysteine synthase from Entamoeba histolytica.

Content Provider	Europe PMC
Author	Krishna, Chinthalapudi Jain, Ruchi Kashav, Tara Wadhwa, Dinakar Alam, Neelima Gourinath, S.
Copyright Year	2007
Description	A preliminary crystallographic study of cysteine synthase, a major enzyme in the cysteine-biosynthesis pathway, from the amoebic pathogen E. histolytica. Entamoeba histolytica, the causative agent of human amoebiasis, is essentially anaerobic, requiring a small amount of oxygen for growth. It cannot tolerate the higher concentration of oxygen present in human tissues or blood. However, during tissue invasion it is exposed to a higher level of oxygen, leading to oxygen stress. Cysteine, which is a vital thiol in E. histolytica, plays an essential role in its oxygen-defence mechanisms. The major route of cysteine biosynthesis in this parasite is the condensation of O-acetylserine with sulfide by the de novo cysteine-biosynthetic pathway, which involves cysteine synthase (EhCS) as a key enzyme. In this study, EhCS was cloned, expressed in Escherichia coli and purified by affinity and size-exclusion chromatography. The purified protein was crystallized in space group P41 with two molecules per asymmetric unit and a complete data set was collected to a resolution of 1.86 Å. A molecular-replacement solution was obtained using the Salmonella typhimurium O-acetylserine sulfhydrylase structure as a probe and had a correlation coefficient of 37.7% and an R factor of 48.8%.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2335080&blobtype=pdf
Volume Number	63
PubMed Central reference number	PMC2335080
Issue Number	Pt 6
Issue Number	pt 6
PubMed reference number	17554175
Journal	Acta Crystallogr Sect F Struct Biol Cryst Commun
e-ISSN	17443091
DOI	10.1107/s1744309107022154
Language	English
Publisher	International Union of Crystallography
Publisher Date	2007-05-12
Access Restriction	Open
Rights License	© International Union of Crystallography 2007
Subject Keyword	cysteine synthase Entamoeba histolytica
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology

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