Loading...
Please wait, while we are loading the content...
Similar Documents
Increased expression of heat shock protein 72 protects renal proximal tubular cells from gentamicin-induced injury.
| Content Provider | Europe PMC |
|---|---|
| Author | Wang, Zhipeng Liu, Li Mei, Qibing Liu, Linna Ran, Yuhua Zhang, Rong |
| Copyright Year | 2006 |
| Abstract | The nephrotoxicity of gentamicin (GM) has been widely recognized. Heat shock protein 72 (HSP72) has been reported to be a cytoprotectant. However, its cytoprotective effect against GM induced kidney injury has not yet been studied. In this study, we investigated the cytoprotective effect of HSP72 on GM-induced nephrotoxicity in vitro. Human Kidney tubular cell line, HK-2 cells were divided into four groups: control group, GM group (cells incubated with GM only), heat shock (HS) group (cells incubated at 43℃ for 30 min), and GM plus HS group, respectively. Lactate dehydrogenanse (LDH) release increased time-dependently from 24 hr to 96 hr compared to the data of cells treated with GM only. Results of NAG activities, superoxide dismutase (SOD) activities and malondialdehyde (MDA) content were similar to that of the LDH release. The amount of HSP72 positive cells increased significartly at 72 hr after cells were treated with GM only. Both HSP72 protein and gene expression increased significantly at 72 hr when cells were treated with GM. On the other hand, HS induced HSP72 expression markedly. Pretreatment of HS inhibited HK-2 cells from GM-induced injury. It could reduce LDH release and NAG activity. HS also increased SOD activity, and decreased MDA content when cells were damaged by GM. These findings suggested that HS may protect kidney cells from GM-induced injury. Pre-induction of HSP72 may provide therapeutic strategies for nephrotoxicity induced by GM. |
| ISSN | 10118934 |
| Volume Number | 21 |
| PubMed Central reference number | PMC2722003 |
| Issue Number | 5 |
| PubMed reference number | 17043427 |
| Journal | Journal of Korean Medical Science [J. Korean Med. Sci] |
| e-ISSN | 15986357 |
| DOI | 10.3346/jkms.2006.21.5.904 |
| Language | English |
| Publisher | The Korean Academy of Medical Sciences |
| Publisher Date | 2006-10-01 |
| Access Restriction | Open |
| Rights License | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2006 The Korean Academy of Medical Sciences |
| Subject Keyword | Gentamicins Drug Toxicity Cytotoxicity Nephrotoxicity Heat-Shock Proteins Kidney Tubules, Proximal |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
