NDLI: Overexpression of the human 72 kDa heat shock protein in renal tubular cells confers resistance against oxidative injury and cisplatin toxicity.

Content Provider	Semantic Scholar
Author	Komatsuda, Atsushi Wakui, Hideki Oyama, Yuko Imai, Hirokazu Miura, Akira B. Itoh, Hiroshi Tashima, Yohtalou
Copyright Year	1999
Abstract	BACKGROUND Recent studies have shown that the 72-kDa heat shock protein (HSP72) can be induced in renal tubular cells by a variety of stress conditions, and suggested its cytoprotective function. We have tested this hypothesis directly by transfection studies. METHODS LLC-PK1 cells (porcine renal tubular epithelial cells) were stably transfected with pBK-CMV or pBK-CMV containing the human HSP72 gene (pBK-CMV-HSP72). These cells were then treated with various concentrations of hydrogen peroxide or cisplatin. The cell viability and lytic cell damage were determined by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and lactate dehydrogenase release assay. RESULTS Immunoblot and immunocytochemical analyses showed the high level expression of HSP72 in LLC-PK1 cells transfected with pBK-CMV-HSP72. In addition, the expression of other major HSPs (HSP90, HSP73, HSP60 and HSP27) was not affected by transfection. LLC-PK1 cells overexpressing HSP72 were significantly more resistant to hydrogen peroxide and cisplatin treatments than control cells. CONCLUSION These results indicate that overexpressed HSP72 plays a direct role in protecting renal tubular cells against oxidative injury and cisplatin toxicity.
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DOI	10.1093/ndt/14.6.1385
Alternate Webpage(s)	https://watermark.silverchair.com/10382997.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAkIwggI-BgkqhkiG9w0BBwagggIvMIICKwIBADCCAiQGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMyxKMGbDgx4zr6_wuAgEQgIIB9ZIdRT5QR1hWZLdPw5VjLEzqM2cFVTsp5jchcYOwoHp2PekckLazX5J6cN4GRm7LfDAeNpkiiRazQnSO7mCf83_BM0Tmc7eG1ox79zUu9BPjNALPphKPDNEFi93qodXjB-rcGVBZCwfsTXa7ZM9-XFBx1DOqKCqVTqHIL9Nb0xtJ3uS-CwtVnZv8IlxTM5Pga1hgpBtzrkgpft3C32LDkB_iLNw_wvVPgpwOATNiVssxZBTNbyH7scehV0TOxVtllIvoDxeOESphoEWMTzzZsMM-LEIQttqUjjaiA1hem64gwAyOCgM6b06qtB9LNKozr7Kv58ekCCKkiFmejPMsB0D-WpWs1bLPYVu6wwM72IkU7jfb0GaNvIqNyOxKCssliipzKL30-LSg1wxp8zwXSXKpNnTF5oh7GQycE9zP50KzZSn_hxnlcz48Uf_LrfGHfVD8tYdmFgHnQn-U7DQyuTHBEplu98ajo2mKEQZGWm0TbOptBKs38YTlVyn4Lni4NWich0eVaR-iZuFTAtHZExP9rxp3RPV8wsqsaMLQOcPn0OJcdaabwpQh6SrX2IiLYJ8uGy7vJm0fEn1x3urvMggyKL2w2tPkCpjb9kRBtVnNMgeQcGojR17ljs-cgpztOMHjM8S9e8pmwDPvF1jpOABhTgmiTQ
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PubMed reference number	10382997
Alternate Webpage(s)	https://doi.org/10.1093/ndt%2F14.6.1385
Journal	Medline
Volume Number	14
Issue Number	6
Journal	Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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