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Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock.
| Content Provider | Europe PMC |
|---|---|
| Author | Lu, Yuan-qiang Cai, Xiu-jun Gu, Lin-hui Mu, Han-zhou Huang, Wei-dong |
| Abstract | Objective: To investigate the potential and early effect of hypertonic saline resuscitation on T-lymphocyte subpopulations in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 18 Sprague-Dawley (SD) rats. The rats were randomly divided into Sham group, HTS group (hypertonic saline resuscitation group) and NS group (normal saline resuscitation group). Each group contained 6 rats. The CD4+ and CD8+ subpopulations of T-lymphocytes in peripheral blood were detected respectively before shock and after resuscitation by double antibody labelling and flow cytometry. Results: In the early stage after hemorrhagic shock, fluid resuscitation and emergency treatment, the CD4+ lymphocytes of peripheral blood in HTS and NS groups markedly increased. Small volume resuscitation with HTS also induced peripheral CD8+ lymphocytes to a certain extent, whereas NS resuscitation showed no effect in this respect. Consequently, compared with Sham and HTS groups, CD4+/CD8+ ratio of peripheral blood in NS group was obviously increased, and showed statistically differences. Conclusion: In this model of rat with severe hemorrhagic shock, small volume resuscitation with HTS is more effective than NS in reducing immunologic disorders and promoting a more balanced profile of T-lymphocyte subpopulations regulating network. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC1764915&blobtype=pdf |
| ISSN | 16731581 |
| Volume Number | 8 |
| PubMed Central reference number | PMC1764915 |
| Issue Number | 1 |
| PubMed reference number | 17173365 |
| Journal | J Zhejiang Univ Sci B |
| DOI | 10.1631/jzus.2007.b0070 |
| Language | English |
| Publisher | Zhejiang University Press |
| Publisher Date | 2007-01-01 |
| Publisher Place | Hangzhou |
| Access Restriction | Open |
| Subject Keyword | Hemorrhagic shock Resuscitation Sodium chloride solution Hypertonic saline T-lymphocyte subpopulations Flow cytometry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics |
