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| Content Provider | Springer Nature Link |
|---|---|
| Author | Lu, Yuan qiang Cai, Xiu jun Gu, Lin hui Mu, Han zhou Huang, Wei dong |
| Copyright Year | 2007 |
| Abstract | Objective: To investigate the potential and early effect of hypertonic saline resuscitation on T-lymphocyte subpopulations in rats with hemorrhagic shock.Methods: A model of rat with severe hemorrhagic shock was established in 18 Sprague-Dawley (SD) rats. The rats were randomly divided into Sham group, HTS group (hypertonic saline resuscitation group) and NS group (normal saline resuscitation group). Each group contained 6 rats. The CD4$^{+}$ and CD8$^{+}$ subpopulations of T-lymphocytes in peripheral blood were detected respectively before shock and after resuscitation by double antibody labelling and flow cytometry.Results: In the early stage after hemorrhagic shock, fluid resuscitation and emergency treatment, the CD4$^{+}$ lymphocytes of peripheral blood in HTS and NS groups markedly increased. Small volume resuscitation with HTS also induced peripheral CD8$^{+}$ lymphocytes to a certain extent, whereas NS resuscitation showed no effect in this respect. Consequently, compared with Sham and HTS groups, CD4$^{+}$/CD8$^{+}$ ratio of peripheral blood in NS group was obviously increased, and showed statistically differences.Conclusion: In this model of rat with severe hemorrhagic shock, small volume resuscitation with HTS is more effective than NS in reducing immunologic disorders and promoting a more balanced profile of T-lymphocyte subpopulations regulating network. |
| Starting Page | 70 |
| Ending Page | 75 |
| Page Count | 6 |
| File Format | |
| ISSN | 16731581 |
| Journal | Journal of Zhejiang University SCIENCE B |
| Volume Number | 8 |
| Issue Number | 1 |
| e-ISSN | 18621783 |
| Language | English |
| Publisher | Zhejiang University Press |
| Publisher Date | 2007-01-01 |
| Publisher Place | Hangzhou |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Hemorrhagic shock Resuscitation Sodium chloride solution Hypertonic saline T-lymphocyte subpopulations Flow cytometry Biomedicine general |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics |
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