NDLI: A Magnetic-Bead-Based Immunoassay with a Newly Developed Monoclonal Antibody for Rapid and Highly Sensitive Detection of Forchlorfenuron.

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A Magnetic-Bead-Based Immunoassay with a Newly Developed Monoclonal Antibody for Rapid and Highly Sensitive Detection of Forchlorfenuron.

Content Provider	Europe PMC
Author	Shan, Yubao He, Ting Li, Ying Zhu, Jiang Yue, Xiali Yang, Yunhuang
Copyright Year	2023
Abstract	Forchlorfenuron (CPPU) is a widely used plant growth regulator in agriculture, and CPPU residue in food can cause harm to human health. Thus, it is necessary to develop a rapid and sensitive detection method for CPPU monitoring. In this study, a new monoclonal antibody (mAb) against CPPU with high affinity was prepared by a hybridoma technique, and a magnetic bead (MB)-based analytical method was established for the determination of CPPU by a one-step procedure. Under optimized conditions, the detection limit of the MB-based immunoassay was as low as 0.0004 ng/mL, which was five times more sensitive than the traditional indirect competitive ELISA (icELISA). In addition, the detection procedure took less than 35 min, a significant improvement over the 135 min required for icELISA. The selectivity test of the MB-based assay also showed negligible cross-reactivity with five analogues. Furthermore, the accuracy of the developed assay was assessed by the analysis of spiked samples, and the results agreed well with those obtained by HPLC. The excellent analytical performance of the proposed assay suggests its great potential for routine screening of CPPU, and it provides a basis for promoting the application of more immunosensors in the quantitative detection of low concentrations of small organic molecules in food.
Volume Number	13
PubMed Central reference number	PMC10296238
Issue Number	6
PubMed reference number	37366958
Journal	Biosensors (Basel)
e-ISSN	20796374
DOI	10.3390/bios13060593
Language	English
Publisher	MDPI
Publisher Date	2023-05-30
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	forchlorfenuron monoclonal antibody magnetic bead detection food safety
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry Instrumentation Medicine Biomedical Engineering Engineering Analytical Chemistry Biotechnology

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