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Detection of Alpha Fetoprotein Based on AIEgen Nanosphere Labeled Aptamer Combined with Sandwich Structure of Magnetic Gold Nanocomposites.
| Content Provider | Europe PMC |
|---|---|
| Author | Liu, Lei Wang, Huixing Sulemana, Husseini Xie, Bing Gao, Li |
| Copyright Year | 2023 |
| Abstract | As a biomarker, alpha-fetoprotein (AFP) is valuable for detecting some tumors in men, non-pregnant women, and children. However, the detection sensitivity in some methods needs to be improved. Therefore, developing a simple, reliable, and sensitive detection method for AFP is important for non-malignant diseases. An aptamer binding was developed based on aggregation-induced emission luminogen (AIEgen) nanosphere labeled with Fe3O4@MPTMS@AuNPs. AFP was detected with a sandwich structure of AuNPs magnetic composite particles. An aggregation-induced emission (AIE) molecule and polystyrene (PS) nanosphere complex were assembled, enhancing the fluorescence and improving the sensitivity of detection. The limit of detection (LOD) was at a given level of 1.429 pg/mL, which can best be achieved in serum samples. Finally, the results obtained showed the complex to be promising in practical applications. |
| Volume Number | 13 |
| PubMed Central reference number | PMC10046738 |
| Issue Number | 3 |
| PubMed reference number | 36979562 |
| Journal | Biosensors (Basel) |
| e-ISSN | 20796374 |
| DOI | 10.3390/bios13030351 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2023-03-06 |
| Access Restriction | Open |
| Rights License | Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors. |
| Subject Keyword | aptamer AFP detection AIE |
| Content Type | Text |
| Resource Type | Article |
| Subject | Clinical Biochemistry Instrumentation Medicine Biomedical Engineering Engineering Analytical Chemistry Biotechnology |
