A Colorimetric Aptasensor for Ochratoxin A Detection Based on Tetramethylrhodamine Charge Effect-Assisted Silver Enhancement.

Content Provider	Europe PMC
Author	Yang, Xiaoyan Huang, Rong Xiong, Lulu Chen, Feng Sun, Wei Yu, Ling
Copyright Year	2023
Abstract	A novel colorimetric aptasensor based on charge effect-assisted silver enhancement was developed to detect ochratoxin A (OTA). To achieve this objective, gold nanoparticles (AuNPs), which can catalyze silver reduction and deposition, were used as the carrier of the aptamers tagged with a positively charged tetramethylrhodamine (TAMRA). Due to the mutual attraction of positive and negative charges, the TAMRA attracted and retained the silver lactate around the AuNPs. Thus, the chance of AuNP-catalyzed silver reduction was increased. The charge effect-assisted silver enhancement was verified by tagging different base pair length aptamers with TAMRA. Under optimized conditions, the as-prepared OTA aptasensor had a working range of 1 × 102–1 × 106 pg mL−1. The detection limit was as low as 28.18 pg mL−1. Moreover, the proposed aptasensor has been successfully applied to determine OTA in actual samples with satisfactory results.
Volume Number	13
PubMed Central reference number	PMC10136965
Issue Number	4
PubMed reference number	37185543
Journal	Biosensors (Basel)
e-ISSN	20796374
DOI	10.3390/bios13040468
Language	English
Publisher	MDPI
Publisher Date	2023-04-10
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	aptamer ochratoxin A gold nanoparticle silver enhancement colorimetric aptasensor
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry Instrumentation Medicine Biomedical Engineering Engineering Analytical Chemistry Biotechnology