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Metallothionein 3 Inhibits 3T3-L1 Adipocyte Differentiation via Reduction of Reactive Oxygen Species.
| Content Provider | Europe PMC |
|---|---|
| Author | Li, Yuankuan Lee, Sung Ho Piao, Meiyu Kim, Hyung Sik Lee, Kwang Youl |
| Editor | Muntean, Danina M. Ro, Seung-Hyun |
| Copyright Year | 2023 |
| Abstract | Metallothionein 3 (MT3), also known as a neuronal growth-inhibitory factor, is a member of the metallothionein family and is involved in a variety of biological functions, including protection against metal toxicity and reactive oxygen species (ROS). However, less is known about the role of MT3 in the differentiation of 3T3-L1 cells into adipocytes. In this study, we observed that MT3 levels were downregulated during 3T3-L1 adipocyte differentiation. Mt3 overexpression inhibited adipocyte differentiation and reduced the levels of the adipogenic transcription factors C/EBPα and PPARγ. Further analyses showed that MT3 also suppressed the transcriptional activity of PPARγ, and this effect was not mediated by a direct interaction between MT3 with PPARγ. In addition, Mt3 overexpression resulted in a decrease in ROS levels during early adipocyte differentiation, while treatment with antimycin A, which induces ROS generation, restored the ROS levels. Mt3 knockdown, on the other hand, elevated ROS levels, which were suppressed upon treatment with the antioxidant N-acetylcysteine. Our findings indicate a previously unknown role of MT3 in the differentiation of 3T3-L1 cells into adipocytes and provide a potential novel target that might facilitate obesity treatment. |
| Journal | Antioxidants (Basel, Switzerland) |
| Volume Number | 12 |
| PubMed Central reference number | PMC10045306 |
| Issue Number | 3 |
| PubMed reference number | 36978888 |
| e-ISSN | 20763921 |
| DOI | 10.3390/antiox12030640 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2023-03-04 |
| Access Restriction | Open |
| Rights License | Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors. |
| Subject Keyword | metallothionein 3 adipocyte differentiation reactive oxygen species PPARγ |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Cell Biology Molecular Biology Physiology Clinical Biochemistry Food Science |
