NDLI: N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation

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N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation

Content Provider	Scilit
Author	Soto, Daniela Gomez-Serrano, María Pieralisi, Azul Calvo, Juan C. Peral, Belén Guerra, Liliana N.
Copyright Year	2016
Description	Objectives: Reports investigating the effects of antioxidants on obesity have provided contradictory results. We have previously demonstrated that treatment with the antioxidant N-acetylcysteine (NAC) inhibits cellular triglyceride (Tg) accumulation as well as total cellular monoamine oxidase A (MAOA) expression in 3T3-L1 mature adipocytes (Calzadilla et al., Redox Rep. 2013;210–218). Here we analyzed the role of NAC on adipogenic differentiation pathway. Methods: Assays were conducted using 3T3-L1 preadipocytes (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC). We studied the effects of different doses of NAC (0.01 or 1 mM) on DC, to evaluate cellular expression of phospho-JNK½ (pJNK½), phospho-ERK½ (pERK½) and, mitochondrial expression of citrate synthase, fumarate hydratase and MAOA. Results: Following the differentiation of preadipocytes, an increase in the expression levels of pJNK½ and pERK½ was observed, together with mitotic clonal expansion (MCE). We found that both doses of NAC decreased the expression of pJNK½ and pERK½. Consistent with these results, NAC significantly inhibited MCE and modified the expression of different mitochondrial proteins. Discussion: Our results suggested that NAC could inhibit Tg and mitochondrial protein expression by preventing both MCE and kinase phosphorylation.
Related Links	https://www.tandfonline.com/doi/pdf/10.1080/13510002.2016.1223267?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837516/pdf
Ending Page	271
Page Count	7
Starting Page	265
ISSN	13510002
e-ISSN	17432928
DOI	10.1080/13510002.2016.1223267
Journal	Redox Report
Issue Number	6
Volume Number	22
Language	English
Publisher	Informa UK Limited
Publisher Date	2016-09-27
Access Restriction	Open
Subject Keyword	Nutrition and Dietetics Antioxidant Obesity 3t3-l1 Adipocyte Differentiation Mitocondria Kinase Phosphorylation Monoamine Oxidase A
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Cell Biology Physiology Biochemistry Clinical Biochemistry

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