Loading...
Please wait, while we are loading the content...
Similar Documents
Splice site proximity influences alternative exon definition
| Content Provider | eScholarship Repository: University of California |
|---|---|
| Author | Carranza, Francisco Shenasa, Hossein Hertel, Klemens J |
| Abstract | Alternative splicing enables higher eukaryotes to expand mRNA diversity from a finite number of genes through highly combinatorial splice site selection mechanisms that are influenced by the sequence of competing splice sites, cis-regulatory elements binding trans-acting factors, the length of exons and introns harbouring alternative splice sites and RNA secondary structures at putative splice junctions. To test the hypothesis that the intron definition or exon definition modes of splice site recognition direct the selection of alternative splice patterns, we created a database of alternative splice site usage (ALTssDB). When alternative splice sites are embedded within short introns (intron definition), the 5' and 3' splice sites closest to each other across the intron preferentially pair, consistent with previous observations. However, when alternative splice sites are embedded within large flanking introns (exon definition), the 5' and 3' splice sites closest to each other across the exon are preferentially selected. Thus, alternative splicing decisions are influenced by the intron and exon definition modes of splice site recognition. The results demonstrate that the spliceosome pairs splice sites that are closest in proximity within the unit of initial splice site selection. |
| File Format | |
| DOI | 10.1080/15476286.2022.2089478 |
| Alternate Webpage(s) | https://escholarship.org/uc/item/1tf4d408 |
| Journal | RNA Biology |
| Issue Number | 1 |
| Volume Number | 19 |
| Language | English |
| Publisher Date | 2022-12-31 |
| Access Restriction | Open |
| Rights License | https://creativecommons.org/licenses/by/4.0/ |
| Subject Keyword | Exon Definition Splice Site Selection Bioinformatics Molecular Biology Biological Sciences Bioinformatics and Computational Biology Genetics Alternative Splicing Exons Introns RNA Splice Sites RNA Splicing Intron-exon architecture Developmental Biology Biochemistry and cell biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology |
