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Bio-functionalized Pt Nanoparticles Based Electrochemical Impedance Immunosensor For Human Cardiac Myoglobin
| Content Provider | CSIR - National Physical Laboratory (NPL) |
|---|---|
| Author | Mishra, Sujeet K. Srivastava, Avanish K. Kumar, Devendra Rajesh |
| Abstract | We report the covalent immobilization of three-dimensional carboxyl-functionalized Pt(MPA) nanoparticles with myoglobin protein antibody by carbodiimide coupling reaction deposited onto an indium-tin-oxide-coated glass plate for the construction of a bioelectrode. This bioelectrode assembly was characterized by spectro microscopic and electrochemical techniques. Electrochemical impedance studies of the bioelectrode showed significant changes in charge transfer resistance (R-et), predominantly in the low AC frequency region of 40 Hz, on immunoreaction with human cardiac myoglobin antigen (Ag-cMb). Ag-cMb detection in phosphate buffer exhibited a linear range of 0.01 mu g mL(-1) to 1 mu g mL(-1) with a sensitivity of 184.8 Omega cm(2) per decade |
| Related Links | http://npl.csircentral.net/1189/1/48.pdf |
| Starting Page | 21267 |
| Ending Page | 21276 |
| Page Count | 10 |
| File Format | |
| ISSN | 20462069 |
| Volume Number | 4 |
| Issue Number | 41 |
| Journal | RSC Advances |
| Language | English |
| Publisher | Royal Society Of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Chemistry Materials Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemical Engineering Chemistry |