NDLI: Pt Nanoparticles-chemical Vapor Deposited Graphene Composite Based Immunosensor For The Detection Of Human Cardiac Troponin

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Pt Nanoparticles-chemical Vapor Deposited Graphene Composite Based Immunosensor For The Detection Of Human Cardiac Troponin

Content Provider	CSIR - National Physical Laboratory (NPL)
Author	Singal, Shobhita Srivastava, Avanish K. Biradar, Ashok M. Mulchandani, Ashok Rajesh
Abstract	We report a sensitive and stable electrochemical impedance immunosensor prepared with functionalized Pt nanoparticles decorated graphene monolayer deposited on a glassy carbon electrode (GCE). Graphene monolayer was grown on a copper foil by chemical vapor deposition (CVD) method using acetylene as a precursor source and transferred it on GCE and electrochemically oxidized to electroactive graphene (EG). 3-Mercaptopropionic acid (MPA) functionalized Pt-nanoparticles, Pt (MPA), were attached to electroactive graphene (EG) using a molecular bilinker 1-pyrenemethylamine (PMA). Pt (MPA) NP offers free terminal carboxyl group for the site specific covalent biomolecular immobilization of troponin antibody, anti-cTnI, through carbodiimide coupling reaction to form an anti-cTnI-Pt(MPA)-PMA EG GCE bioelectrode. The bioelectrode was characterized by transmission electron microscopy, scanning electron microscopy, Raman spectroscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The bioelectrode exhibited a linear electrochemical impedance response to cTnI over the concentration range of 0.01-10 ng mL-1 in phosphate buffer saline (pH 7.4) with a sensitivity of 80 SZ cm2 per decade and a limit of detection of 4.2 pgmL(-1) cTnI.
Related Links	http://npl.csircentral.net/1486/1/327.pdf
Starting Page	363
Ending Page	370
Page Count	8
File Format	PDF
ISSN	09254005
Volume Number	205.
Journal	Sensors and Actuators B: Chemical
Language	English
Publisher	Elsevier
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chemistry Electrochemistry Instruments/ Instrumentation
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Surfaces, Coatings and Films Electronic, Optical and Magnetic Materials Instrumentation Condensed Matter Physics Electrical and Electronic Engineering Materials Chemistry

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