NDLI: Application of Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis to the Characterization of Ricin A-Chain Immunotoxins

Content Provider	Springer Nature Link
Author	Na, Dong Hee Park, Eun Ji Kim, Myung Sun Lee, Hye Suk Lee, Kang Choon
Copyright Year	2012
Abstract	This study was undertaken to use sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) for the characterization of immunotoxins constructed by cross-linking ricin A-chain (RTA) to monoclonal antibody (MAb). SDS-CGE could separate mono- and di-RTA-conjugated MAbs from unreacted MAb, as identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Resolution of RTA-MAb conjugates was improved by decreasing the running voltage from 15 to 5 kV. SDS-CGE was also useful for characterizing aggregates of RTA or deglycosylated RTA (dgRTA) that was formed during immunotoxin preparation. Of the RTA isomers with different carbohydrate contents, RTA1, which has low carbohydrate content, was found to be more susceptible to aggregation than RTA2. In the SDS-CGE of dgRTA, both covalently and non-covalently linked aggregates were observed under reducing and non-reducing conditions. The findings of this study suggest that SDS-CGE can be used to optimize methods of preparing effective immunotoxins and for checking degradants and aggregates.
Starting Page	679
Ending Page	683
Page Count	5
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	75
Issue Number	11-12
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-04-20
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sodium dodecyl sulfate-capillary gel electrophoresis Immunotoxins Monoclonal antibody Ricin Pharmacy Proteomics Laboratory Medicine Chromatography
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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