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The Neuroactive Peptide N-Acetylaspartylglutamate Is Not an Agonist at the Metabotropic Glutamate Receptor Subtype 3 of Metabotropic Glutamate Receptor." J Pharmacol Exp Ther 330.1 (2009)
| Content Provider | CiteSeerX |
|---|---|
| Author | Johnson, Edwin C. Hampson, David R. Blake, Timothy J. Yao, Yi Chopra, Maninder |
| Abstract | The peptide N-acetylaspartylglutamate (NAAG) is present in high concentrations in the mammalian central nervous system. Various mechanisms have been proposed for its action, includ-ing selective activation of the metabotropic glutamate receptor (mGluR) subtype 3, its action at the N-methyl-D-aspartate re-ceptor, or the production of glutamate by its hydrolysis cata-lyzed by an extracellular protease. To re-examine its agonist activity at mGluR3, we coexpressed human or rat mGluR3 with G protein inward rectifying channels in Xenopus laevis oocytes. High-performance liquid chromatography analysis of commer-cial sources of NAAG showed 0.38 to 0.48 % glutamate con-tamination. Although both human and rat mGluR3 were highly sensitive to glutamate, with EC50 values of 58 and 28 nM, respectively, purified NAAG (100 M) had little activity (7.7 % of |
| File Format | |
| Publisher Date | 2009-01-01 |
| Access Restriction | Open |
| Content Type | Text |