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Regulation of human organic anion transporter 3 by peptide hormone bradykinin. J Pharmacol Exp Ther
| Content Provider | CiteSeerX |
|---|---|
| Author | Li, Shanshan Duan, Peng You, Guofeng |
| Abstract | Human organic anion transporter (hOAT) 3 belongs to a family of organic anion transporters that play critical roles in the body disposition of numerous clinically important drugs. In the cur-rent study, we examined the regulation of hOAT3 by peptide hormone bradykinin (BK) in COS-7 cells. BK (500 nM) induced a concentration- and time-dependent stimulation of hOAT3 activity, kinetically revealed as an increased Vmax. Such an increase in Vmax resulted from an increased cell surface expres-sion without a change in total cell expression of the transporter. BK-induced stimulation of hOAT3 activity could be prevented by treating hOAT3-expressing cells with staurosporine, a gen-eral inhibitor for protein kinase C (PKC). To obtain further infor-mation on which PKC isoform mediates BK regulation of hOAT3 activity, cellular distribution of various PKC isoforms |
| File Format | |
| Access Restriction | Open |
| Content Type | Text |