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Whole-cell sensing for a harmful bloom-forming microscopic alga by measuring antibody-antigen forces (2006)
| Content Provider | CiteSeerX |
|---|---|
| Author | Lee, Er S. Mahapatro, Mrinal Caron, David A. Requicha, Aristides A. G. Stauffer, Beth A. Thompson, Mark E. Zhou, Chongwu |
| Abstract | Abstract—Aureococcus anophagefferens, a harmful bloom-forming alga responsible for brown tides in estuaries of the Middle Atlantic U.S., has been investigated by atomic force microscopy for the first time, using probes functionalized with a monoclonal antibody specific for the alga. The rupture force between a single monoclonal antibody and the surface of A. anophagefferens was experimentally found to be 246 11 pN at the load rate of 12 nN/s. Force histograms for A. anophagefferens and other similarly-sized algae are presented and analyzed. The results illustrate the effects of load rates, and demonstrate that force-distance measurements can be used to build biosensors with high signal-to-noise ratios for A. anophagefferens. The methods described in this paper can be used, in principle, to construct sensors with single-cell resolution for arbitrary cells for which monoclonal antibodies are available. Index Terms—Atomic force microscopy, Aureococcus anophagef-ferens, biosensors, force-distance measurements, single-cell identi-fication. I. |
| File Format | |
| Journal | IEEE Trans. Nanobioscience |
| Language | English |
| Publisher Date | 2006-01-01 |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
