NDLI: Whole-Cell Sensing for a Harmful Bloom-Forming Microscopic Alga by Measuring Antibody

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Issue 2

Issue 1

Year : 2005 Volume : 4

Year : 2004 Volume : 3

Year : 2003 Volume : 2

Year : 2002 Volume : 99

Whole-Cell Sensing for a Harmful Bloom-Forming Microscopic Alga by Measuring Antibody–Antigen Forces

Content Provider	IEEE Xplore Digital Library
Author	Lee, A.S. Mahapatro, M. Caron, D.A. Requicha, A.A.G. Stauffer, B.A. Thompson, M.E. Chongwu Zhou
Copyright Year	2002
Abstract	Aureococcus anophagefferens, a harmful bloom-forming alga responsible for brown tides in estuaries of the Middle Atlantic U.S., has been investigated by atomic force microscopy for the first time, using probes functionalized with a monoclonal antibody specific for the alga. The rupture force between a single monoclonal antibody and the surface of A. anophagefferens was experimentally found to be 246 plusmn 11 pN at the load rate of 12 nN/s. Force histograms for A. anophagefferens and other similarly-sized algae are presented and analyzed. The results illustrate the effects of load rates, and demonstrate that force-distance measurements can be used to build biosensors with high signal-to-noise ratios for A. anophagefferens. The methods described in this paper can be used, in principle, to construct sensors with single-cell resolution for arbitrary cells for which monoclonal antibodies are available
Sponsorship	IEEE Engineering in Medicine and Biology Society IEEE Systems, Man, and Cybernetics Society IEEE Nanotechnology Council IEEE Computational Intelligence Society IEEE Robotics and Automation Society IEEE Computer Society
Page Count	8
File Size	1408051
Starting Page	149
Ending Page	156
File Format	PDF
ISSN	15361241
Volume Number	5
Issue Number	3
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-09-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Force measurement Atomic force microscopy Biosensors Tides Atomic measurements Probes Histograms Algae Signal to noise ratio Sensor phenomena and characterization single-cell identification Aureococcus anophagefferens biosensors force-distance measurements
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Bioengineering Electrical and Electronic Engineering Biomedical Engineering Computer Science Applications Biotechnology Pharmaceutical Science

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Whole-Cell Sensing for a Harmful Bloom-Forming Microscopic Alga by Measuring Antibody-Antigen Forces

Whole-cell sensing for a harmful bloom-forming microscopic alga by measuring antibody--antigen forces (2006)

Whole-cell sensing for a harmful bloom-forming microscopic alga by measuring antibody-antigen forces (2006)

Transport of the harmful bloom alga aureococcus anophagefferens by oceangoing ships and coastal boats (2004).

A Brown Tide Bloom Index based on the potential harmful effects of the brown tide alga , Aureococcus anophagefferens

Transport of the harmful bloom alga Aureococcus anophagefferens by oceangoing ships and coastal boats.

Transport of the harmful bloom alga Aureococcus anophagefferens by oceangoing ships and coastal boats.

Niche of harmful alga Aureococcus anophagefferens revealed through ecogenomics

Alga aureococcus anophagefferens.

Whole-Cell Sensing for a Harmful Bloom-Forming Microscopic Alga by Measuring Antibody–Antigen Forces

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Whole-Cell Sensing for a Harmful Bloom-Forming Microscopic Alga by Measuring Antibody-Antigen Forces

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Whole-cell sensing for a harmful bloom-forming microscopic alga by measuring antibody-antigen forces (2006)

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Whole-Cell Sensing for a Harmful Bloom-Forming Microscopic Alga by Measuring Antibody&#8211;Antigen Forces

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