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The structure of the yeast plasma membrane snare complex reveals destabilizing water filled cavities (2008).
| Content Provider | CiteSeerX |
|---|---|
| Author | Strop, Pavel Kaiser, Stephen E. Vrljic, Marija Brunger, Axel T. |
| Abstract | SNARE proteins form a complex that leads to membrane fusion between vesicles, organelles, and plasmamembrane in all eukaryotic cells. We report the 1.7 A ̊ resolution structure of the SNARE complex that mediates exocytosis at the plasma mem-brane in the yeast Saccharomyces cerevisiae. Similar to its neu-ronal and endosomal homologues, the S. cerevisiae SNARE complex forms a parallel four-helix bundle in the center of which is an ionic layer. The S. cerevisiae SNARE complex exhib-its increased helix bending near the ionic layer, contains water-filled cavities in the complex core, and exhibits reduced thermal stability relative to mammalian SNARE complexes. Mutagene-sis experiments suggest that the water-filled cavities contribute to the lower stability of the S. cerevisiae complex. Membrane fusion is a fundamental and highly regulated process that is required for the transport of proteins, lipids, and |
| File Format | |
| Publisher Date | 2008-01-01 |
| Access Restriction | Open |
| Subject Keyword | Water-filled Cavity Ionic Layer Plasma Mem-brane Resolution Structure Snare Complex Yeast Saccharomyces Cerevisiae Parallel Four-helix Bundle Thermal Stability Mutagene-sis Experiment Cerevisiae Snare Complex Exhib-its Cerevisiae Complex Complex Core Mammalian Snare Complex Membrane Fusion Eukaryotic Cell Cerevisiae Snare Complex Form Regulated Process Helix Bending Endosomal Homologues |
| Content Type | Text |
