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Regulation of TCR-mediated T cell activation by TNF-RII
| Content Provider | CiteSeerX |
|---|---|
| Author | Eibl, Martha Marianne Wolf, Hermann Maximilian Aspalter, Rosa Maria |
| Abstract | Abstract: In the present study, we investigated the role of tumor necrosis factor receptor II (TNF-RII) in human T cell activation induced via the T cell receptor (TCR) in an antigen-presenting cell-independent system. Our results confirm that in-teraction of TNF- with TNF-RII but not TNF-RI is directly costimulatory to TCR-mediated T cell ac-tivation, thereby augmenting T cell proliferation, expression of T cell activation markers (CD25, human leukocyte antigen-DR, TNF-RII), and se-cretion of cytokines such as interferon- and TNF-. In contrast to the well-defined costimula-tory molecule CD28, costimulation via TNF-RII showed significant differences in kinetics, require-ment for cross-linking, redundancy of intracellular signaling pathways involved, and the capacity to induce interleukin (IL)-2, IL-10, and IL-13 secre-tion. In addition, cross-linking TNF-RII had the capacity to down-regulate TCR/CD28-induced Ca mobilization, IL-2 mRNA expression, and IL-2 and IL-10 secretion. Taken together, our findings demonstrate that TNF-RII plays a unique role among the T cell costimulatory molecules, as TNF-RII ligation can have positive and negative effects on TCR-dependent signaling. TNF-RII cross-linking has an inhibitory effect on early TCR signaling events proximal to induction of Ca flux, which ultimately leads to modulation of the T cell cytokine pattern expressed. J. Leukoc. Biol. |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Ca Flux Human Cell Activation Tnf-rii Ligation Cell Proliferation Down-regulate Tcr Cd28-induced Ca Mobilization Negative Effect Tnf-rii Cross-linking Antigen-presenting Cell-independent System Inhibitory Effect Significant Difference Unique Role Il-10 Secretion Cell Cytokine Pattern Tcr-dependent Signaling Well-defined Costimula-tory Molecule Cd28 Tcr-mediated Cell Activation Cell Activation Marker Cross-linking Tnf-rii Early Tcr Tcr-mediated Cell Ac-tivation Il-2 Mrna Expression Tumor Necrosis Factor Receptor Ii Cell Receptor Human Leukocyte Antigen-dr Il-13 Secre-tion Cell Costimulatory Molecule |
| Content Type | Text |
| Resource Type | Article |
