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Expression of cd28 and cd86 by human eosinophils and role in the secretion of type 1 cytokines (interleukin 2 and interferon.
| Content Provider | CiteSeerX |
|---|---|
| Author | Complexes, Inhibition Immunoglobulin A. Woerly, Gaëtane Roger, Nadine Loiseau, Sylvie Dombrowicz, David Capron, André Capron, Monique |
| Abstract | Eosinophils are the source of various immunoregulatory cytokines, but the membrane molecules involved in their secretion have not been clearly identified. Here we show that peripheral blood eosinophils from hypereosinophilic patients could express membrane CD86 but not CD80. The T cell costimulatory molecule CD28 is also detected on the eosinophil surface. CD28 ligation but not CD86 ligation resulted in interleukin (IL)-2 and interferon (IFN)- � secretion by eosinophils, whereas IL-4, IL-5, and IL-10 were not detected. In contrast to T cells requiring two signals for effective stimulation, CD28 ligation alone was sufficient for optimal eosinophil activation. Eosinophil-derived IL-2 and IFN- � were biologically active, as supernatants from anti-CD28–treated cells were able to induce CTLL-2 proliferation and major histocompatibility complex class II expression on the colon carcinoma cell line Colo 205, respectively. Addition of secretory immunoglobulin (Ig)A–anti-IgA complexes, which could induce the release of IL-10, very significantly inhibited both CD28-mediated IL-2 and IFN- � release. These results suggest that the release of type 1 (IFN- � and IL-2) versus type 2 cytokines by eosinophils is not only differential but also dependent on cross-regulatory signals. They confirm that through activation of costimulatory molecules, eosinophils could function as an immunoregulatory |
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| Access Restriction | Open |
| Subject Keyword | Human Eosinophil Cd28 Ligation Membrane Cd86 Cell Costimulatory Molecule Cd28 Optimal Eosinophil Activation Cross-regulatory Signal Costimulatory Molecule Ifn Release Hypereosinophilic Patient Effective Stimulation Eosinophil Surface Colon Carcinoma Cell Line Colo Anti-iga Complex Cd86 Ligation Membrane Molecule Peripheral Blood Eosinophil Anti-cd28 Treated Cell Cd28-mediated Il-2 Various Immunoregulatory Cytokine Secretory Immunoglobulin Ctll-2 Proliferation |
| Content Type | Text |
