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Protein Microarray On-Demand: A Novel Protein Microarray System (2008)
| Content Provider | CiteSeerX |
|---|---|
| Author | Sitaraman, Kalavathy Hartley, James Baptista, Cassio Hill, Thomas M. Chatterjee, Deb K. Munroe, David J. |
| Abstract | We describe a novel, simple and low-cost protein microarray strategy wherein the microarrays are generated by printing expression ready plasmid DNAs onto slides that can be converted into protein arrays on-demand. The printed expression plasmids serve dual purposes as they not only direct the synthesis of the protein of interest; they also serve to capture the newly synthesized proteins through a high affinity DNA-protein interaction. To accomplish this we have exploited the highaffinity binding (,3–7610 213 M) of E. coli Tus protein to Ter, a 20 bp DNA sequence involved in the regulation of E. coli DNA replication. In our system, each protein of interest is synthesized as a Tus fusion protein and each expression construct directing the protein synthesis contains embedded Ter DNA sequence. The embedded Ter sequence functions as a capture reagent for the newly synthesized Tus fusion protein. This ‘‘all DNA’ ’ microarray can be converted to a protein microarray |
| File Format | |
| Publisher Date | 2008-01-01 |
| Access Restriction | Open |
| Subject Keyword | Expression Ready Plasmid Dna Coli Tus Protein Protein Synthesis Novel Protein Microarray System Low-cost Protein Microarray Strategy High Affinity Dna-protein Interaction Bp Dna Sequence Dna Microarray Embedded Ter Sequence Function Protein Microarray On-demand Printed Expression Plasmid Expression Construct Protein Array On-demand Protein Microarray Dual Purpose Ter Dna Sequence Highaffinity Binding Tus Fusion Protein Coli Dna Replication Capture Reagent |
| Content Type | Text |
