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Neurite Fasciculation Mediated by Complexes of Axonin-1 and Ng Cell Adhesion Molecule
| Content Provider | CiteSeerX |
|---|---|
| Author | Kunz, Stefan Sonderegger, Peter Buchstaller, Andrea Ginsburg, Claudia Lanz, Rainer Spirig, Marianne Kunz, Beat Vogt, Lorenz Rader, Christoph |
| Abstract | Abstract. Neural cell adhesion molecules composed of immunoglobulin and fibronectin type III-like domains have been implicated in cell adhesion, neurite outgrowth, and fasciculation. Axonin-1 and Ng cell adhesion molecule (NgCAM), two molecules with predominantly axonal expression exhibit homophilic interactions across the extracellular space (axonin-1/axonin-1 and NgCAM/NgCAM) and a heterophilic interaction (axonin-1–NgCAM) that occurs exclusively in the plane of the same membrane (cis-interaction). Using domain deletion mutants we localized the NgCAM homophilic binding in the Ig domains 1-4 whereas heterophilic binding to axonin-1 was localized in the Ig domains 2-4 and the third FnIII domain. The NgCAM–NgCAM interaction could be established simultaneously with the axonin-1–NgCAM interaction. In contrast, the axonin-1–NgCAM interaction excluded axonin-1/axonin-1 binding. These results and the examination of the coclustering of axonin-1 and NgCAM at cell contacts, suggest that intercellular contact is mediated by a symmetric axonin-1 2/NgCAM 2 tetramer, in which homophilic NgCAM binding across the extracellular space occurs simultaneously with a cis-heterophilic interaction of axonin-1 and NgCAM. The enhanced neurite fasciculation after overexpression of NgCAM by adenoviral vectors indicates that NgCAM is the limiting component for the formation of the axonin-1 2/NgCAM 2 complexes and, thus, neurite fasciculation in DRG neurons. Key words: axonal fasciculation • axon guidance molecules • cell adhesion molecules • cell contact • cell recognition The precise wiring of the neuronal network is essential for the proper information-processing function of the nervous system. During the formation of the basic network neurons send out processes that reach their target regions in a highly stereotyped and precise manner and very few errors of navigation occur (Dodd and Jessell, |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Axonin-1 Axonin-1 Binding Nervous System 1-4 Whereas Neurite Fasciculation Ngcam Ngcam Interaction Cell Contact Extracellular Space Basic Network Neuron Third Fniii Domain Target Region Enhanced Neurite Fasciculation Axonin-1 Ngcam Interaction Proper Information-processing Function Ngcam Homophilic Binding Axonin-1 Axonin-1 Heterophilic Interaction Neuronal Network Navigation Occur Axonal Fasciculation Axon Guidance Molecule Adenoviral Vector Neurite Fasciculation Mediated Cis-heterophilic Interaction Homophilic Ngcam Binding Drg Neuron Ng Cell Adhesion Molecule Adhesion Molecule Intercellular Contact Neural Cell Adhesion Molecule Neurite Outgrowth Ngcam Ngcam Cell Adhesion Axonal Expression Exhibit Homophilic Interaction Domain Deletion Mutant Contact Cell Recognition Fibronectin Type Iii-like Domain Precise Wiring Precise Manner Limiting Component Axonin-1 Ngcam |
| Content Type | Text |
| Resource Type | Article |
