NDLI: Optimization of Geometry for Continuous Flow PCR Devices in a Titer Plate-Based PCR Multi-Reactor Platform

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Park, D. S. Chen, P. -C You, B. H. Kim, N. Park, T. Datta, P. Desta, Y. Soper, S. A. Nikitopoulos, D. E. Murphy, M. C.
Copyright Year	2007
Abstract	A highly parallel, polymerase chain reaction (PCR) multireactor platform is in high demand to satisfy the high throughput requirements for exploiting the accumulated genetic information from the Human Genome Project. By incorporating continuous flow PCR (CFPCR) devices in a polymer 96-well titer plate format, DNA amplification can be performed with steady-state temperature control and faster reaction speed at lower cost. Prior to the realization of a PCR multi-reactor platform, consisting of a sample delivery chip, a PCR multireactor chip, and a thermal cycler, optimization of the geometry for CFPCR devices in a titer plate-based PCR multi-reactor chip based on manufacturing feasibility is necessary. A prototype PCR multi-reactor chip was designed in a 96-well titer plate format with twelve different CFPCR configurations. High quality metallic, large area mold inserts (LAMIs) were fabricated using an SU-8 based UV-LIGA technique by overplating nickel in SU-8 electroplating templates. Micro molding of polycarbonate (PC) was done using hot embossing, resulting in good replication fidelity over the large surface area. Thermal fusion bonding of the molded PC chips using a custom-made bonding jig yielded acceptable sealing results. The manufacturability investigation throughout the design and the process sequence suggested that the microchannel walls require a minimum width of at least 20 μm and an aspect ratio of 2 for structural rigidity. An optimal CFPCR device for use in a PCR multi-reactor chip can be selected with a series of amplification experiments with the development of a thermal cycler.
Starting Page	113
Ending Page	118
Page Count	6
File Format	PDF
ISBN	079184305X
DOI	10.1115/IMECE2007-42135
e-ISBN	0791838129
Volume Number	Volume 11: Micro and Nano Systems, Parts A and B
Conference Proceedings	ASME 2007 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2007-11-11
Publisher Place	Seattle, Washington, USA
Access Restriction	Subscribed
Subject Keyword	Chain Molding Embossing Sealing (process) Electroplating Flow (dynamics) Steady state Optimization Microchannels Geometry Design Ultraviolet radiation Dna Jigs and fixtures Nickel Stiffness Manufacturing Temperature control Polymers Bonding Engineering prototypes
Content Type	Text
Resource Type	Article

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