NDLI: Chondrocyte Translocation Response to Direct Current Electric Fields

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Pen-Hsiu Grace Chao Roy, Rani Mauck, Robert L. Liu, Wendy Valhmu, Wilmot B. Hung, Clark T.
Copyright Year	2000
Abstract	Using a custom galvanotaxis chamber and time-lapse digital video microscopy, we report the novel observation that cultured chondrocytes exhibit cathodal migration when subjected to applied direct current (DC) electric fields as low as 0.8 V/cm. The response was dose-dependent for field strengths greater than 4 V/cm. Cell migration appeared to be an active process with extension of cytoplasmic processes in the direction of movement. In some cells, field application for greater than an hour induced elongation of initially round cells accompanied by perpendicular alignment of the long axis with respect to the applied field. Antagonists of the inositol phospholipid pathway, U-73122 and neomycin, were able to inhibit cathodal migration. Cell migration toward the cathode did not require the presence of serum during field application. However, the directed velocity was nearly threefold greater in studies performed with serum. Studies performed at physiologic temperatures (∼37°C) revealed a twofold enhancement in migration speed compared to similar studies at room temperature (∼25°C). Findings from the present study may help to elucidate basic mechanisms that mediate chondrocyte migration and substrate attachment. Since chondrocyte migration has been implicated in cartilage healing, the ability to direct chondrocyte movement has the potential to impact strategies for addressing cartilage healing/repair and for development of cartilage substitutes. [S0148-0731(00)00803-7]
Starting Page	261
Ending Page	267
Page Count	7
File Format	PDF
ISSN	01480731
e-ISSN	15288951
Journal	Journal of Biomechanical Engineering
Volume Number	122
Issue Number	3
DOI	10.1115/1.429661
Language	English
Publisher	The American Society of Mechanical Engineers
Publisher Date	2000-02-06
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cartilage Chondrocytes Electric fields Maintenance Mechanisms Temperature
Content Type	Text
Resource Type	Article
Subject	Physiology (medical) Biomedical Engineering

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