NDLI: lambda DNA Fingerprinting Simulation

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lambda DNA Fingerprinting Simulation

Content Provider	howtosmile
Author	Conley, Thomas J.
Copyright Year	2009
Description	The purpose of this lab activity is to demonstrate (through simulation) how DNA fingerprinting (or DNA profiling) might be used to solve a crime. Learners perform restriction digests on DNA samples from four individuals, and then search for similarities between the individuals by running the restriction fragments on an electrophoresis gel. This activity does not do a true DNA fingerprint. It simulates two of the three steps of DNA fingerprinting: restriction of DNA sample and separation by electrophoresis. This activity does not make use of the third step, the radioactive probes. In order to make DNA fingerprinting affordable, lambda DNA is used instead of plasmids. This means that the instructor has to switch the labels on the samples given to the learners. What is labeled DNA is actually the different restriction enzymes and what is labeled restriction enzyme is the lambda DNA. Although there is some deception on the part of the instructor, learners are able to do a restriction digest that simulates a crime scene which adds interest for the learner.
Related Links	http://www.accessexcellence.org/AE/AEC/AEF/1996/conley_dna.html
Language	English
Access Restriction	Open
Rights Holder	Access Excellence @ the National Health Museum
Subject Keyword	DNA DNA profiling Fingerprinting Crime Electrophoresis Restriction enzymes Recombinant DNA Forensics Crime Science Life Sciences Cells Chemistry of Life Heredity and Genetics DNA Structure and Function Physical Sciences Chemistry The Nature of Science The Scientific Process Conducting Investigations Gathering Data Formulating Explanations Communicating Results
Content Type	Text
Educational Role	Student Teacher
Educational Use	Assessment
Time Required	PT4H0M0S
Education Level	Class IX Class X Class XI Class XII
Pedagogy	Experimental Activity
Resource Type	Hands-on
Subject	Biology

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