| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Osterholzer, John J. Moore, Bethany B. Przybranowski, Sally Zaslona, Zbigniew Wilkinson, John E. Teitz-Tennenbaum, Seagal Wilke, Carol Van Rooijen, Nico Peters-Golden, Marc |
| Description |
Author Affiliation: Zaslona Z ( Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109); Przybranowski S ( Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109); Wilke C ( Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109); van Rooijen N ( Department of Molecular Cell Biology, VU Medical Center, Amsterdam 1081 HV, the Netherlands); Teitz-Tennenbaum S ( Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109); Osterholzer JJ ( Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109); Wilkinson JE ( Unit for Laboratory Animal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109); Moore BB ( Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109); Peters-Golden M ( Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109) |
| Abstract |
The role and origin of alveolar macrophages (AMs) in asthma are incompletely defined. We sought to clarify these issues in the context of acute allergic lung inflammation using house dust mite and OVA murine models. Use of liposomal clodronate to deplete resident AMs (rAMs) resulted in increased levels of inflammatory cytokines and eosinophil numbers in lavage fluid and augmented the histopathologic evidence of lung inflammation, suggesting a suppressive role for rAMs. Lung digests of asthmatic mice revealed an increased percentage of Ly6C(high)/CD11b(pos) inflammatory monocytes. Clodronate depletion of circulating monocytes, by contrast, resulted in an attenuation of allergic inflammation. A CD45.1/CD45.2 chimera model demonstrated that recruitment at least partially contributes to the AM pool in irradiated nonasthmatic mice, but its contribution was no greater in asthma. Ki-67 staining of AMs supported a role for local proliferation, which was increased in asthma. Our data demonstrate that rAMs dampen, whereas circulating monocytes promote, early events in allergic lung inflammation. Moreover, maintenance of the AM pool in the early stages of asthmatic inflammation depends on local proliferation, but not recruitment. |
| ISSN |
00221767 |
| e-ISSN |
15506606 |
| DOI |
10.4049/jimmunol.1400580 |
| Journal |
The Journal of Immunology |
| Issue Number |
8 |
| Volume Number |
193 |
| Language |
English |
| Publisher |
The American Association of Immunologists |
| Publisher Date |
2014-10-15 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Asthma Immunology Inflammation Macrophages, Alveolar Monocytes Allergens Alveolitis, Extrinsic Allergic Animals Antigens, Cd11b Biosynthesis Antigens, Cd45 Genetics Antigens, Ly Bronchoalveolar Lavage Fluid Cytology Cell Proliferation Clodronic Acid Pharmacology Cytokines Disease Models, Animal Eosinophils Lung Pathology Mice Mice, Inbred C57bl Ovalbumin Pneumonia Pyroglyphidae Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Immunology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Immunology and Allergy Immunology |
