| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Doorenspleet, Marieke de Vries, Niek Van Schaik, Barbera Klarenbeek, Paul Van Kampen, Antoine Moody, D. Branch Van Rhijn, Ildiko |
| Description |
Author Affiliation: van Schaik B ( Bioinformatics Laboratory, Department of Clinical Epidemiology, Biostatistics, and Bioinformatics, Academic Medical Center, University of Amsterdam, 1100 DD Amsterdam, the Netherlands); Klarenbeek P ( Department of Clinical Immunology and Rheumatology, Academic Medical Center, University of Amsterdam, 1100 DD Amsterdam, the Netherlands); Doorenspleet M ( Department of Clinical Immunology and Rheumatology, Academic Medical Center, University of Amsterdam, 1100 DD Amsterdam, the Netherlands); van Kampen A ( Bioinformatics Laboratory, Department of Clinical Epidemiology, Biostatistics, and Bioinformatics, Academic Medical Center, University of Amsterdam, 1100 DD Amsterdam, the Netherlands); Moody DB ( Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115); de Vries N ( Department of Clinical Immunology and Rheumatology, Academic Medical Center, University of Amsterdam, 1100 DD Amsterdam, the Netherlands); Van Rhijn I ( Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115) |
| Abstract |
During infection and autoimmune disease, activation and expansion of T cells take place. Consequently, the TCR repertoire contains information about ongoing and past diseases. Analysis and interpretation of the human TCR repertoire are hampered by its size and stochastic variation and by the diversity of Ags and Ag-presenting molecules encoded by the MHC, but are highly desirable and would greatly impact fundamental and clinical immunology. A subset of the TCR repertoire is formed by invariant T cells. Invariant T cells express interdonor-conserved TCRs and recognize a limited set of Ags, presented by nonpolymorphic Ag-presenting molecules. Discovery of the three known invariant T cell populations has been a tedious and slow process, identifying them one by one. Because conservation of the TCR -chain of invariant T cells is much higher than the ß-chain, and because the TCR -chain V gene segment TRAV1-2 is used by two of the three known invariant TCRs, we employed next-generation sequencing of TCR -chains that contain the TRAV1-2 gene segment to identify 16 invariant TCRs shared among many blood donors. Frequency analysis of individual clones indicates these T cells are expanded in many donors, implying an important role in human immunity. This approach extends the number of known interdonor-conserved TCRs and suggests that many more exist and that these TCR patterns can be used to systematically evaluate human Ag exposure. |
| ISSN |
00221767 |
| e-ISSN |
15506606 |
| DOI |
10.4049/jimmunol.1401380 |
| Journal |
The Journal of Immunology |
| Issue Number |
10 |
| Volume Number |
193 |
| Language |
English |
| Publisher |
The American Association of Immunologists |
| Publisher Date |
2014-11-15 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Latent Tuberculosis Genetics Receptors, Antigen, T-cell, Alpha-beta T-lymphocyte Subsets Immunology Amino Acid Sequence Asymptomatic Diseases Clone Cells Conserved Sequence Gene Expression Hla Antigens High-throughput Nucleotide Sequencing Immunophenotyping Microbiology Pathology Molecular Sequence Data Protein Isoforms Chemistry Classification Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Immunology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Immunology and Allergy Immunology |
