| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Blom, Kim Braun, Monika Falconer, Karolin Fogdell-Hahn, Anna Michaëlsson, Jakob Ivarsson, Martin A. Gonzalez, Veronica D. Marquardt, Nicole Gustafsson, Rasmus Sandberg, Johan K. |
| Description |
Author Affiliation: Marquardt N ( Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden); Ivarsson MA ( Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden); Blom K ( Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden); Gonzalez VD ( Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden); Braun M ( Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden); Falconer K ( Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden); Gustafsson R ( Department of Clinical Neuroscience, Multiple Sclerosis Research Group, Center for Molecular Medicine, Karolinska Institutet, 171 77 Stockholm, Sweden.); Fogdell-Hahn A ( Department of Clinical Neuroscience, Multiple Sclerosis Research Group, Center for Molecular Medicine, Karolinska Institutet, 171 77 Stockholm, Sweden.); Sandberg JK ( Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden); Michaëlsson J ( Department of Medicine, Center for Infectious Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden) |
| Abstract |
NK cells play an important role in the defense against viral infections. However, little is known about the regulation of NK cell responses during the first days of acute viral infections in humans. In this study, we used the live attenuated yellow fever virus (YFV) vaccine 17D as a human in vivo model to study the temporal dynamics and regulation of NK cell responses in an acute viral infection. YFV induced a robust NK cell response in vivo, with an early activation and peak in NK cell function at day 6, followed by a delayed peak in Ki67 expression, which was indicative of proliferation, at day 10. The in vivo NK cell response correlated positively with plasma type I/III IFN levels at day 6, as well as with the viral load. YFV induced an increased functional responsiveness to IL-12 and IL-18, as well as to K562 cells, indicating that the NK cells were primed in vivo. The NK cell responses were associated primarily with the stage of differentiation, because the magnitude of induced Ki67 and CD69 expression was distinctly higher in CD57(-) NK cells. In contrast, NK cells expressing self- and nonself-HLA class I-binding inhibitory killer cell Ig-like receptors contributed, to a similar degree, to the response. Taken together, our results indicate that NK cells are primed by type I/III IFN in vivo early after YFV infection and that their response is governed primarily by the differentiation stage, independently of killer cell Ig-like receptor/HLA class I-mediated inhibition or education. |
| ISSN |
00221767 |
| e-ISSN |
15506606 |
| Journal |
The Journal of Immunology |
| Issue Number |
7 |
| Volume Number |
195 |
| Language |
English |
| Publisher |
The American Association of Immunologists |
| Publisher Date |
2015-10-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Interferon Type I Immunology Killer Cells, Natural Receptors, Kir Yellow Fever Vaccine Yellow Fever Virus Antibodies, Neutralizing Antigens, Cd Biosynthesis Antigens, Cd57 Metabolism Antigens, Differentiation, T-lymphocyte B-lymphocytes Cell Differentiation Cell Proliferation Histocompatibility Antigens Class I Blood Interleukin-12 Subunit P35 Interleukin-18 K562 Cells Ki-67 Antigen Cytology Lectins, C-type Lymphocyte Activation T-lymphocytes Vaccines, Attenuated Viral Load Viral Vaccines Research Support, Non-u.s. Gov't Discipline Immunology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Immunology and Allergy Immunology |
