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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Carlock, L. R. Dana, S. L. Wasmuth, J. J. Skarecky, D. |
| Abstract | A complete genomic DNA library was prepared from a Chinese hamster-human cell hybrid that contains human chromosome 5 as its only human DNA. Unique or low-copy DNA fragments, isolated form recombinant bacteriophage that contained human DNA inserts, were regionally mapped on chromosome 5 using Southern blot analysis of genomic DNA from a series of hybrid cell lines that were selected as having deletions of various portions of 5q. The chromosome 5-specific DNA library, together with a genetic selective procedure allowing the isolation of hybrid cell lines with deletions of virtually any portion of 5q, will provide a means to construct very accurate physical and recombinational maps of this human chromosome. This system represents an excellent opportunity to examine very precisely the relationship between physical and genetic distances for many loci along the length of this autosome. |
| ISSN | 00029297 |
| e-ISSN | 15376605 |
| Journal | The American Journal of Human Genetics |
| Issue Number | 5 |
| Volume Number | 37 |
| Language | English |
| Publisher | Cell Press (on behalf of American Society of Human Genetics) |
| Publisher Date | 1985-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Chromosome Deletion Chromosome Mapping Chromosomes, Human, 4-5 Animals Cell Line Chromosome Banding Cricetinae Cricetulus Dna Restriction Enzymes Dna, Recombinant Electrophoresis, Agar Gel Genetic Markers Hybrid Cells Karyotyping Ovary Research Support, U.s. Gov't, P.h.s. Discipline Human Genetics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Genetics (clinical) |
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