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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Miller, W. L. Mohandas, T. K. Picado-Leonard, J. Wu, D. A. Morel, Y. Chang, C. Y. Chung, B. C. |
| Description | Author Affiliation: Morel Y ( Department of Pediatrics, University of California, San Francisco 94143.) |
| Abstract | Adrenodoxin is a small iron/sulfur protein serving as an electron-transport intermediate for all mitochondrial forms of cytochrome P450. Southern blots of normal genomic DNA cleaved with six restriction endonucleases probed with full-length human adrenodoxin cDNA revealed complex patterns indicating the presence of multiple adrenodoxin genes. Southern blots of DNA from a panel of mouse/human somatic cell hybrids identified cross-hybridizing adrenodoxin DNA in two loci, chromosome 11q13----qter and chromosome 20cen----q13.1. Examination of adrenodoxin clones from a genomic DNA library in phage lambda revealed some clones bearing gene fragments interrupted by introns and other clones bearing processed pseudogenes. By probing the mouse/human hybrids with unique intronic DNA and by correlating restriction maps of the phage clones with that of uncloned genomic DNA, we show that the authentic transcribed adrenodoxin gene lies on chromosome 11, while pseudogenes lie on chromosome 20. |
| ISSN | 00029297 |
| e-ISSN | 15376605 |
| Journal | The American Journal of Human Genetics |
| Issue Number | 1 |
| Volume Number | 43 |
| Language | English |
| Publisher | Cell Press (on behalf of American Society of Human Genetics) |
| Publisher Date | 1988-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Adrenodoxin Genetics Chromosome Mapping Chromosomes, Human, Pair 11 Chromosomes, Human, Pair 20 Genes Pseudogenes Dna Restriction Enzymes Genetic Markers Nucleic Acid Hybridization Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, P.h.s. Discipline Human Genetics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Genetics (clinical) |
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