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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Serjeantson, S. W. Gatenby, P. A. Jazwinska, E. C. Dunckley, H. Propert, D. N. |
| Description | Country affiliation: Australia Author Affiliation: Jazwinska EC ( John Curtin School of Medical Research, Canberra, Australia.) |
| Abstract | This study was undertaken to investigate a means of assigning Gm allotypes to Caucasians by RFLP analysis. A single immunoglobulin heavy-chain gamma-4 cDNA probe (HU gamma 4) was hybridized with genomic DNA digested separately with two restriction enzymes, TaqI and PvuII. Results showed excellent correlation (P less than .001) between serologically defined Gm allotypes G1m(1), G1m(2), G2m(23), and G1m;G3m (3;5,10) and RFLPs identified with the (HU gamma 4) probe. We conclude that it is now possible to define common Gm haplotypes in Caucasians by RFLP analysis. This method provides a useful adjunct to serological allotyping and indeed has several important advantages over traditional serology: it allows confident Gm assignment and the definition of homozygous and heterozygous Gm arrangements, is highly reproducible, and is readily executed in any molecular genetic laboratory. |
| ISSN | 00029297 |
| e-ISSN | 15376605 |
| Journal | The American Journal of Human Genetics |
| Issue Number | 2 |
| Volume Number | 43 |
| Language | English |
| Publisher | Cell Press (on behalf of American Society of Human Genetics) |
| Publisher Date | 1988-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Deoxyribonucleases, Type Ii Site-specific Genes, Immunoglobulin Immunoglobulin Heavy Chains Genetics Polymorphism, Genetic Polymorphism, Restriction Fragment Length Cloning, Molecular Dna Restriction Enzymes Nucleic Acid Hybridization Research Support, Non-u.s. Gov't Discipline Human Genetics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Genetics (clinical) |
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