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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Milatovich, A. Kitamura, T. Francke, U. Miyajima, A. |
| Description | Country affiliation: Canada Author Affiliation: Milatovich A ( Department of Genetics, Stanford University School of Medicine, CA.) |
| Abstract | Interleukin-3 (IL3) and granulocyte/macrophage colony-stimulating factor (CSF2) stimulate proliferation and differentiation of various hemopoietic cell types. As is characteristic of the cytokine receptor family, the receptors for these proteins comprise alpha- and beta-subunits. While IL3 and CSF2 receptors each have unique alpha- subunits, they share a common beta-subunit. By Southern analysis of somatic cell hybrid panels, pulsed-field gel electrophoresis (PFGE), and fluorescence chromosomal in situ hybridization, we have mapped the cloned sequence for the IL3 receptor alpha (IL3RA) to the X-Y pseudoautosomal region at bands Xp22.3 and Yp11.3, near the gene for the alpha-subunit of the CSF2 receptor (CSF2RA). The CSF2RA and IL3RA genes are so close that their order could not be determined by two-color interphase in situ hybridization. They share PFGE fragments generated by different restriction enzymes down to the 50-100-kb size range. Pseudoautosomal inheritance was demonstrated by an EcoRI RFLP detected with the IL3RA cDNA probe. |
| ISSN | 00029297 |
| e-ISSN | 15376605 |
| Journal | The American Journal of Human Genetics |
| Issue Number | 5 |
| Volume Number | 53 |
| Language | English |
| Publisher | Cell Press (on behalf of American Society of Human Genetics) |
| Publisher Date | 1993-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Receptors, Interleukin-3 Genetics X Chromosome Y Chromosome Animals Blotting, Southern Chromosome Mapping Chromosomes, Human Cloning, Molecular Cricetinae Electrophoresis, Gel, Pulsed-field Hybrid Cells In Situ Hybridization, Fluorescence Pedigree Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, P.h.s. Discipline Human Genetics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Genetics (clinical) |
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